• Login
    View Item 
    •   UMB Digital Archive
    • School, Graduate
    • Theses and Dissertations All Schools
    • View Item
    •   UMB Digital Archive
    • School, Graduate
    • Theses and Dissertations All Schools
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UMB Digital ArchiveCommunitiesPublication DateAuthorsTitlesSubjectsThis CollectionPublication DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    Display statistics

    Development and implementation of theoretical models for molecular scale distance distributions for comparison with fluorescence resonance energy transfer measurements

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Find Full text
    Author
    Fishman, Mayer Nahman
    Advisor
    Lakowicz, Joseph R.
    Date
    1993
    Type
    dissertation
    
    Metadata
    Show full item record
    Abstract
    The objective of this project is to develop theoretical calculations of molecular scale distance distributions for comparison with those detectable with time resolved fluorescence spectroscopy measurements, emphasizing the frequency domain technique. Fluorescence resonance energy transfer (FRET) measurements are in principle sensitive to donor-acceptor (D-A) distances and distance distribution functions. Heterogeneity of emission rates is interpreted as heterogeneity of D-to-A energy transfer rates and D-to-A distances. The goal of uniting the theoretical and experimental domains is advanced from the theoretical side by two sets of computer programs. The first program is an implementation of the rotational isomeric state (RIS) model as described by Flory (1969) for the end-to-end distance distribution functions of chain-like molecules. Application of the algorithm to alkyl-linker molecules is presented, as well as some work on peptide-linker molecules. Using this program this independent perspective on the shape of the end-to-end distance distribution function is shown to reinforce the shape of a parameterized one recovered from the data. Conversely the RIS model is shown to be consistent with the fluorescence data, for the longer alkyl molecules described here. The second program is an implementation of a Monte Carlo integration over the space of possible donor-acceptors configurations, or Explicit Fluorophore Distribution Simulation (EFDS). In it, models for the distribution functions available to energy transfer acceptors may be tested, with decreased restrictions of assumed symmetry or uniformity of kinetic patterns. The method is shown to be consistent by comparison with solutions based on geometries which are amenable to analytic solutions. This is followed by examples of behavior based on some restrictive geometries. The programs provide an improved mechanism for comparing models described in geometric terms with values from the experimental domain. In so doing, the applicability of the models can be evaluated. It is hoped that quantification of molecular flexibility and configurational heterogeneity will improve the interpretation and utility of fluorescence experiments and ultimately lead to a better understanding of molecular behavior at this scale.
    Description
    University of Maryland, Baltimore. Ph.D. 1993
    Keyword
    Chemistry, Biochemistry
    Physics, Molecular
    Computer Science
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/1648
    Collections
    Theses and Dissertations All Schools
    Theses and Dissertations School of Medicine

    entitlement

     
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Policies | Contact Us | UMB Health Sciences & Human Services Library
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.