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dc.contributor.authorArad, Michal
dc.contributor.authorBrown, Robert A
dc.contributor.authorKhatri, Raju
dc.contributor.authorTaylor, Rodney J
dc.contributor.authorZalzman, Michal
dc.date.accessioned2021-08-24T18:49:20Z
dc.date.available2021-08-24T18:49:20Z
dc.date.issued2021-08-18
dc.identifier.urihttp://hdl.handle.net/10713/16459
dc.description.abstractBackground: Neurological disorders are considered one of the greatest burdens to global public health and a leading cause of death. Stem cell therapies hold great promise for the cure of neurological disorders, as stem cells can serve as cell replacement, while also secreting factors to enhance endogenous tissue regeneration. Adult human multipotent stem cells (MSCs) reside on blood vessels, and therefore can be found in many tissues throughout the body, including palatine tonsils. Several studies have reported the capacity of MSCs to differentiate into, among other cell types, the neuronal lineage. However, unlike the case with embryonic stem cells, it is unclear whether MSCs can develop into mature neurons. Methods: Human tonsillar MSCs (T-MSCs) were isolated from a small, 0.6-g sample, of tonsillar biopsies with high viability and yield as we recently reported. Then, these cells were differentiated by a rapid, multi-stage procedure, into committed, post-mitotic, neuron-like cells using defined conditions. Results: Here we describe for the first time the derivation and differentiation of tonsillar biopsy-derived MSCs (T-MSCs), by a rapid, multi-step protocol, into post-mitotic, neuron-like cells using defined conditions without genetic manipulation. We characterized our T-MSC-derived neuronal cells and demonstrate their robust differentiation in vitro. Conclusions: Our procedure leads to a rapid neuronal lineage commitment and loss of stemness markers, as early as three days following neurogenic differentiation. Our studies identify biopsy-derived T-MSCs as a potential source for generating neuron-like cells which may have potential use for in vitro modeling of neurodegenerative diseases or cell replacement therapies. © 2021, The Author(s).en_US
dc.description.urihttps://doi.org/10.1186/s11658-021-00279-4en_US
dc.language.isoenen_US
dc.publisherSpringer Natureen_US
dc.relation.ispartofCellular & Molecular Biology Lettersen_US
dc.rights© 2021. The Author(s).en_US
dc.subjectBiopsiesen_US
dc.subjectDifferentiationen_US
dc.subjectNeuroblastsen_US
dc.subjectNeuron-like cellsen_US
dc.subjectNeuronal stem cells (NSC)en_US
dc.subjectTonsil-derived multipotent stem cells (T-MSC)en_US
dc.titleDirect differentiation of tonsillar biopsy-derived stem cells to the neuronal lineageen_US
dc.typeArticleen_US
dc.identifier.doi10.1186/s11658-021-00279-4
dc.identifier.pmid34407767
dc.source.volume26
dc.source.issue1
dc.source.beginpage38
dc.source.endpage
dc.source.countryUnited States
dc.source.countryEngland


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