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dc.contributor.authorChiu, Sufen
dc.date.accessioned2012-06-15T19:31:32Z
dc.date.available2012-06-15T19:31:32Z
dc.date.issued1993
dc.identifier.urihttp://hdl.handle.net/10713/1639
dc.descriptionUniversity of Maryland, Baltimore. Ph.D. 1993en_US
dc.description.abstractChanges in behavior and neuroendocrine secretions in response to prolactin treatment may be mediated by prolactin receptors in the brain. Autoradiographic binding studies using iodinated prolactin suggest that these receptors may be located in the rat choroid plexus and hypothalamus. It remains unclear if the prolactin binding sites represent classical prolactin receptors or other proteins from the prolactin/growth hormone receptor family that have retained an affinity for prolactin. Two prolactin receptors, a short and long form resulting from alternative splicing of one gene, were cloned recently. The objectives of this dissertation were to (1) examine if the prolactin receptor gene is expressed in brain areas that are believed to bind and/or respond to prolactin by reverse-transcription polymerase chain reaction (RT-PCR), (2) determine the cellular distribution of prolactin receptor mRNA by in situ hybridization, and (3) assess the influence of physiological conditions on prolactin receptor gene expression at the individual cell level by in situ hybridization. We demonstrated that both forms of the prolactin receptor mRNA are expressed in the pituitary gland and hypothalamus but not in cortical brain tissue or skeletal muscle by RT-PCR. Using in situ hybridization, prolactin receptor gene expression was detectable in medial preoptic nucleus, supraoptic nucleus, arcuate nucleus, lateral ventromedial nucleus and choroid plexus. Prolactin receptor gene expression in the periventricular area of the preoptic nucleus, arcuate nucleus, and choroid plexus is increased during aging {dollar}(p<0.05){dollar}. Therefore, we conclude that the classical prolactin receptors appear to mediate the binding and action of prolactin in the brain and are influenced by age-related changes.en_US
dc.language.isoen_USen_US
dc.subjectBiology, Molecularen_US
dc.subjectBiology, Neuroscienceen_US
dc.subjectBiology, Animal Physiologyen_US
dc.subject.meshAgingen_US
dc.subject.meshHypothalamusen_US
dc.subject.meshProlactinen_US
dc.subject.meshReceptors, Prolactin--geneticsen_US
dc.titleProlactin receptor gene expression in the hypothalamusen_US
dc.typedissertationen_US
dc.contributor.advisorWise, Phyllis M.
dc.identifier.ispublishedYes
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