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dc.contributor.authorTvedte, Eric S
dc.contributor.authorMichalski, Jane
dc.contributor.authorCheng, Shaoji
dc.contributor.authorPatkus, Rayanna S
dc.contributor.authorTallon, Luke J
dc.contributor.authorSadzewicz, Lisa
dc.contributor.authorBruno, Vincent M
dc.contributor.authorSilva, Joana C
dc.contributor.authorRasko, David A
dc.contributor.authorDunning Hotopp, Julie C
dc.date.accessioned2021-08-11T12:11:20Z
dc.date.available2021-08-11T12:11:20Z
dc.date.issued2021-08-05
dc.identifier.urihttp://hdl.handle.net/10713/16359
dc.description.abstractLibrary preparation for high-throughput sequencing applications is a critical step in producing representative, unbiased sequencing data. The iGenomX Riptide High Throughput Rapid Library Prep Kit purports to provide high-quality sequencing data with lower costs compared to other Illumina library kits. To test these claims, we compared sequence data quality of Riptide libraries to libraries constructed with KAPA Hyper and NEBNext Ultra. Across several single-source genome samples, mapping performance and de novo assembly of Riptide libraries were similar to conventional libraries prepared with the same DNA. Poor performance of some libraries resulted in low sequencing depth. In particular, degraded DNA samples may be challenging to sequence with Riptide. There was little cross-well plate contamination with the overwhelming majority of reads belong to the proper source genomes. The sequencing of metagenome samples using different Riptide primer sets resulted in variable taxonomic assignment of reads. Increased adoption of the Riptide kit will decrease library preparation costs. However, this method might not be suitable for degraded DNA.en_US
dc.description.urihttps://doi.org/10.1038/s41598-021-94911-0en_US
dc.language.isoenen_US
dc.publisherSpringer Natureen_US
dc.relation.ispartofScientific Reportsen_US
dc.rights© 2021. The Author(s).en_US
dc.subjectiGenomX Riptide High Throughput Rapid Library Prep Kiten_US
dc.subject.meshEvaluation Studyen_US
dc.subject.meshHigh-Throughput Nucleotide Sequencingen_US
dc.titleEvaluation of a high-throughput, cost-effective Illumina library preparation kiten_US
dc.typeArticleen_US
dc.identifier.doi10.1038/s41598-021-94911-0
dc.identifier.pmid34354114
dc.source.volume11
dc.source.issue1
dc.source.beginpage15925
dc.source.endpage
dc.source.countryUnited States
dc.source.countryEngland


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