Evaluation of a high-throughput, cost-effective Illumina library preparation kit
AuthorTvedte, Eric S
Patkus, Rayanna S
Tallon, Luke J
Bruno, Vincent M
Silva, Joana C
Rasko, David A
Dunning Hotopp, Julie C
MetadataShow full item record
AbstractLibrary preparation for high-throughput sequencing applications is a critical step in producing representative, unbiased sequencing data. The iGenomX Riptide High Throughput Rapid Library Prep Kit purports to provide high-quality sequencing data with lower costs compared to other Illumina library kits. To test these claims, we compared sequence data quality of Riptide libraries to libraries constructed with KAPA Hyper and NEBNext Ultra. Across several single-source genome samples, mapping performance and de novo assembly of Riptide libraries were similar to conventional libraries prepared with the same DNA. Poor performance of some libraries resulted in low sequencing depth. In particular, degraded DNA samples may be challenging to sequence with Riptide. There was little cross-well plate contamination with the overwhelming majority of reads belong to the proper source genomes. The sequencing of metagenome samples using different Riptide primer sets resulted in variable taxonomic assignment of reads. Increased adoption of the Riptide kit will decrease library preparation costs. However, this method might not be suitable for degraded DNA.
Rights/Terms© 2021. The Author(s).
KeywordiGenomX Riptide High Throughput Rapid Library Prep Kit
High-Throughput Nucleotide Sequencing
Identifier to cite or link to this itemhttp://hdl.handle.net/10713/16359
- Optimization and cost-saving in tagmentation-based mate-pair library preparation and sequencing.
- Authors: Tatsumi K, Nishimura O, Itomi K, Tanegashima C, Kuraku S
- Issue date: 2015 May
- A comparative analysis of library prep approaches for sequencing low input translatome samples.
- Authors: Song Y, Milon B, Ott S, Zhao X, Sadzewicz L, Shetty A, Boger ET, Tallon LJ, Morell RJ, Mahurkar A, Hertzano R
- Issue date: 2018 Sep 21
- Optimization of enzymatic fragmentation is crucial to maximize genome coverage: a comparison of library preparation methods for Illumina sequencing.
- Authors: Ribarska T, Bjørnstad PM, Sundaram AYM, Gilfillan GD
- Issue date: 2022 Feb 1
- A performance evaluation of Nextera XT and KAPA HyperPlus for rapid Illumina library preparation of long-range mitogenome amplicons.
- Authors: Ring JD, Sturk-Andreaggi K, Peck MA, Marshall C
- Issue date: 2017 Jul
- MinION™ nanopore sequencing of environmental metagenomes: a synthetic approach.
- Authors: Brown BL, Watson M, Minot SS, Rivera MC, Franklin RB
- Issue date: 2017 Mar 1