Evaluation of a high-throughput, cost-effective Illumina library preparation kit
Author
Tvedte, Eric SMichalski, Jane
Cheng, Shaoji
Patkus, Rayanna S
Tallon, Luke J
Sadzewicz, Lisa
Bruno, Vincent M
Silva, Joana C
Rasko, David A
Dunning Hotopp, Julie C
Date
2021-08-05Journal
Scientific ReportsPublisher
Springer NatureType
Article
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Library preparation for high-throughput sequencing applications is a critical step in producing representative, unbiased sequencing data. The iGenomX Riptide High Throughput Rapid Library Prep Kit purports to provide high-quality sequencing data with lower costs compared to other Illumina library kits. To test these claims, we compared sequence data quality of Riptide libraries to libraries constructed with KAPA Hyper and NEBNext Ultra. Across several single-source genome samples, mapping performance and de novo assembly of Riptide libraries were similar to conventional libraries prepared with the same DNA. Poor performance of some libraries resulted in low sequencing depth. In particular, degraded DNA samples may be challenging to sequence with Riptide. There was little cross-well plate contamination with the overwhelming majority of reads belong to the proper source genomes. The sequencing of metagenome samples using different Riptide primer sets resulted in variable taxonomic assignment of reads. Increased adoption of the Riptide kit will decrease library preparation costs. However, this method might not be suitable for degraded DNA.Rights/Terms
© 2021. The Author(s).Keyword
iGenomX Riptide High Throughput Rapid Library Prep KitEvaluation Study
High-Throughput Nucleotide Sequencing
Identifier to cite or link to this item
http://hdl.handle.net/10713/16359ae974a485f413a2113503eed53cd6c53
10.1038/s41598-021-94911-0
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