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    Evaluation of a high-throughput, cost-effective Illumina library preparation kit

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    Author
    Tvedte, Eric S
    Michalski, Jane
    Cheng, Shaoji
    Patkus, Rayanna S
    Tallon, Luke J
    Sadzewicz, Lisa
    Bruno, Vincent M
    Silva, Joana C
    Rasko, David A
    Dunning Hotopp, Julie C
    Date
    2021-08-05
    Journal
    Scientific Reports
    Publisher
    Springer Nature
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.1038/s41598-021-94911-0
    Abstract
    Library preparation for high-throughput sequencing applications is a critical step in producing representative, unbiased sequencing data. The iGenomX Riptide High Throughput Rapid Library Prep Kit purports to provide high-quality sequencing data with lower costs compared to other Illumina library kits. To test these claims, we compared sequence data quality of Riptide libraries to libraries constructed with KAPA Hyper and NEBNext Ultra. Across several single-source genome samples, mapping performance and de novo assembly of Riptide libraries were similar to conventional libraries prepared with the same DNA. Poor performance of some libraries resulted in low sequencing depth. In particular, degraded DNA samples may be challenging to sequence with Riptide. There was little cross-well plate contamination with the overwhelming majority of reads belong to the proper source genomes. The sequencing of metagenome samples using different Riptide primer sets resulted in variable taxonomic assignment of reads. Increased adoption of the Riptide kit will decrease library preparation costs. However, this method might not be suitable for degraded DNA.
    Rights/Terms
    © 2021. The Author(s).
    Keyword
    iGenomX Riptide High Throughput Rapid Library Prep Kit
    Evaluation Study
    High-Throughput Nucleotide Sequencing
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/16359
    ae974a485f413a2113503eed53cd6c53
    10.1038/s41598-021-94911-0
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