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dc.contributor.authorNoel, Gaelle
dc.contributor.authorIn, Julie G
dc.contributor.authorLemme-Dumit, Jose M
dc.contributor.authorDeVine, Lauren R
dc.contributor.authorCole, Robert N
dc.contributor.authorGuerrerio, Anthony L
dc.contributor.authorCampbell, James D
dc.contributor.authorKovbasnjuk, Olga
dc.contributor.authorPasetti, Marcela F
dc.date.accessioned2021-08-03T15:39:19Z
dc.date.available2021-08-03T15:39:19Z
dc.date.issued2021-07-13
dc.identifier.urihttp://hdl.handle.net/10713/16292
dc.description.abstractBreastfeeding has been associated with long lasting health benefits. Nutrients and bioactive components of human breast milk promote cell growth, immune development, and shield the infant gut from insults and microbial threats. The molecular and cellular events involved in these processes are ill defined. We have established human pediatric enteroids and interrogated maternal milk's impact on epithelial cell maturation and function in comparison with commercial infant formula. Colostrum applied apically to pediatric enteroid monolayers reduced ion permeability, stimulated epithelial cell differentiation, and enhanced tight junction function by upregulating occludin. Breast milk heightened the production of antimicrobial peptide α-defensin 5 by goblet and Paneth cells, and modulated cytokine production, which abolished apical release of pro-inflammatory GM-CSF. These attributes were not found in commercial infant formula. Epithelial cells exposed to breast milk elevated apical and intracellular pIgR and enabled maternal IgA translocation. Proteomic data revealed a breast milk-induced molecular pattern associated with tissue remodeling and homeostasis. Using a novel ex vivo pediatric enteroid model, we have identified distinct cellular and molecular events involved in human milk-mediated improvement of human intestinal physiology and immunity.en_US
dc.description.urihttps://doi.org/10.3389/fcell.2021.685171en_US
dc.language.isoenen_US
dc.publisherFrontiers Media S.A.en_US
dc.relation.ispartofFrontiers in Cell and Developmental Biologyen_US
dc.rightsCopyright © 2021 Noel, In, Lemme-Dumit, DeVine, Cole, Guerrerio, Campbell, Kovbasnjuk and Pasetti.en_US
dc.subjectbreastmilken_US
dc.subjectenteroiden_US
dc.subjectepithelial barrieren_US
dc.subjectinnate immunityen_US
dc.subjectoccludinen_US
dc.subjectpIgR polymeric immunoglobulin receptoren_US
dc.subjectpediatric (infant)en_US
dc.titleHuman Breast Milk Enhances Intestinal Mucosal Barrier Function and Innate Immunity in a Healthy Pediatric Human Enteroid Modelen_US
dc.typeArticleen_US
dc.identifier.doi10.3389/fcell.2021.685171
dc.identifier.pmid34327199
dc.source.volume9
dc.source.beginpage685171
dc.source.endpage
dc.source.countrySwitzerland


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