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dc.contributor.authorAlafeef, Maha
dc.contributor.authorMoitra, Parikshit
dc.contributor.authorDighe, Ketan
dc.contributor.authorPan, Dipanjan
dc.date.accessioned2021-07-29T19:07:10Z
dc.date.available2021-07-29T19:07:10Z
dc.date.issued2021-07-19
dc.identifier.urihttp://hdl.handle.net/10713/16270
dc.description.abstractEfficient monitoring of SARS-CoV-2 outbreak requires the use of a sensitive and rapid diagnostic test. Although SARS-CoV-2 RNA can be detected by RT-qPCR, the molecular-level quantification of the viral load is still challenging, time-consuming, and labor-intensive. Here, we report an ultrasensitive hyperspectral sensor (HyperSENSE) based on hafnium nanoparticles (HfNPs) for specific detection of COVID-19 causative virus, SARS-CoV-2. Density functional theoretical calculations reveal that HfNPs exhibit higher changes in their absorption wavelength and light scattering when bound to their target SARS-CoV-2 RNA sequence relative to the gold nanoparticles. The assay has a turnaround time of a few seconds and has a limit of detection in the yoctomolar range, which is 1 000 000-fold times higher than the currently available COVID-19 tests. We demonstrated in ∼100 COVID-19 clinical samples that the assay is highly sensitive and has a specificity of 100%. We also show that HyperSENSE can rapidly detect other viruses such as influenza A H1N1. The outstanding sensitivity indicates the potential of the current biosensor in detecting the prevailing presymptomatic and asymptomatic COVID-19 cases. Thus, integrating hyperspectral imaging with nanomaterials establishes a diagnostic platform for ultrasensitive detection of COVID-19 that can potentially be applied to any emerging infectious pathogen.en_US
dc.description.urihttps://doi.org/10.1021/acsnano.1c05226en_US
dc.language.isoenen_US
dc.publisherAmerican Chemical Societyen_US
dc.relation.ispartofACS Nanoen_US
dc.subjectSARS-CoV-2en_US
dc.subjectantisense oligonucleotidesen_US
dc.subjectdark-field microscopyen_US
dc.subjecthafniumen_US
dc.subjecthyperspectral imagingen_US
dc.titleHyperspectral Mapping for the Detection of SARS-CoV-2 Using Nanomolecular Probes with Yoctomole Sensitivityen_US
dc.typeArticleen_US
dc.identifier.doi10.1021/acsnano.1c05226
dc.identifier.pmid34279093
dc.source.countryUnited States


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