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dc.contributor.authorWang, Duo
dc.contributor.authorQu, Xiujuan
dc.contributor.authorLu, Wenqing
dc.contributor.authorWang, Yizhe
dc.contributor.authorJin, Yue
dc.contributor.authorHou, Kezuo
dc.contributor.authorYang, Bowen
dc.contributor.authorLi, Ce
dc.contributor.authorQi, Jianfei
dc.contributor.authorXiao, Jiawen
dc.contributor.authorChe, Xiaofang
dc.contributor.authorLiu, Yunpeng
dc.date.accessioned2021-07-26T16:18:11Z
dc.date.available2021-07-26T16:18:11Z
dc.date.issued2021-07-01
dc.identifier.urihttp://hdl.handle.net/10713/16215
dc.description.abstractAbnormal RNA m6A methylation is known to lead to the occurrence and progression of multiple cancers including gastric cancer (GC). However, the integrative effects of all m6A methylation regulators on GC prognosis are unclear. Our research aimed to globally analyze the prognosis values of all 33 m6A RNA methylation regulators in GC by univariate and multivariate Cox regression analyses. Among all 33 m6A RNA methylation regulators, fat mass and obesity-associated protein (FTO), an m6A demethylase, was identified as a key prognostic risk factor on overall survival (OS) of GC patients. It was found that FTO could promote GC cell migration and invasion abilities, and we predicted that ITGB1 was a demethylated target of FTO. Knockdown (KD) of FTO significantly down-regulated ITGB1 expression at both mRNA and protein levels and augmented ITGB1 mRNA m6A modification level. Moreover, overexpression (OE) of ITGB1 could partially reverse FTO-KD-inhibited migration and invasion of GC cells. Our study found that FTO was an independent risk factor for overall survival (OS) of GC patients and FTO could promote GC metastasis by upregulating the expression of Integrin β1(ITGB1) via decreasing its m6A level. These results indicated that FTO can be a potent GC biomarker for prognosis prediction as well as a potential target in GC treatment.en_US
dc.description.urihttps://doi.org/10.3389/fonc.2021.681280en_US
dc.language.isoenen_US
dc.publisherFrontiers Media S.A.en_US
dc.relation.ispartofFrontiers in Oncologyen_US
dc.rightsCopyright © 2021 Wang, Qu, Lu, Wang, Jin, Hou, Yang, Li, Qi, Xiao, Che and Liu.en_US
dc.subjectFTOen_US
dc.subjectITGB1en_US
dc.subjectgastric canceren_US
dc.subjectm6Aen_US
dc.subjectmetastasisen_US
dc.titleN 6-Methyladenosine RNA Demethylase FTO Promotes Gastric Cancer Metastasis by Down-Regulating the m6A Methylation of ITGB1en_US
dc.typeArticleen_US
dc.identifier.doi10.3389/fonc.2021.681280
dc.identifier.pmid34277426
dc.source.volume11
dc.source.beginpage681280
dc.source.endpage
dc.source.countrySwitzerland


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