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dc.contributor.authorBrandenburg, Cheryl
dc.contributor.authorSmith, Lindsey A
dc.contributor.authorKilander, Michaela B C
dc.contributor.authorBridi, Morgan S
dc.contributor.authorLin, Yu-Chih
dc.contributor.authorHuang, Shiyong
dc.contributor.authorBlatt, Gene J
dc.date.accessioned2021-07-12T14:09:41Z
dc.date.available2021-07-12T14:09:41Z
dc.date.issued2021-06-28
dc.identifier.urihttp://hdl.handle.net/10713/16171
dc.description.abstractPurkinje cells (PCs) are central to cerebellar information coding and appreciation for the diversity of their firing patterns and molecular profiles is growing. Heterogeneous subpopulations of PCs have been identified that display differences in intrinsic firing properties without clear mechanistic insight into what underlies the divergence in firing parameters. Although long used as a general PC marker, we report that the calcium binding protein parvalbumin labels a subpopulation of PCs, based on high and low expression, with a conserved distribution pattern across the animals examined. We trained a convolutional neural network to recognize the parvalbumin subtypes and create maps of whole cerebellar distribution and find that PCs within these areas have differences in spontaneous firing that can be modified by altering calcium buffer content. These subtypes also show differential responses to potassium and calcium channel blockade, suggesting a mechanistic role for variability in PC intrinsic firing through differences in ion channel composition. It is proposed that ion channels drive the diversity in PC intrinsic firing phenotype and parvalbumin calcium buffering provides capacity for the highest firing rates observed. These findings open new avenues for detailed classification of PC subtypes.en_US
dc.description.urihttps://doi.org/10.1016/j.mcn.2021.103650en_US
dc.language.isoenen_US
dc.publisherElsevier Inc.en_US
dc.relation.ispartofMolecular and Cellular Neurosciencesen_US
dc.rightsCopyright © 2021. Published by Elsevier Inc.en_US
dc.subjectBurst-pauseen_US
dc.subjectCalcium homeostasisen_US
dc.subjectIntrinsic plasticityen_US
dc.subjectParvalbuminen_US
dc.subjectPurkinjeen_US
dc.subjectSpontaneous firingen_US
dc.titleParvalbumin subtypes of cerebellar Purkinje cells contribute to differential intrinsic firing propertiesen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.mcn.2021.103650
dc.identifier.pmid34197921
dc.source.volume115
dc.source.beginpage103650
dc.source.endpage
dc.source.countryUnited States


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