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dc.contributor.authorGoerlich, Corbin E
dc.contributor.authorGriffith, Bartley
dc.contributor.authorSingh, Avneesh K
dc.contributor.authorAbdullah, Mohamed
dc.contributor.authorSingireddy, Shreya
dc.contributor.authorKolesnik, Irina
dc.contributor.authorLewis, Billeta
dc.contributor.authorSentz, Faith
dc.contributor.authorTatarov, Ivan
dc.contributor.authorHershfeld, Alena
dc.contributor.authorZhang, Tianshu
dc.contributor.authorStrauss, Erik
dc.contributor.authorOdonkor, Patrick
dc.contributor.authorWilliams, Brittney
dc.contributor.authorTabatabai, Ali
dc.contributor.authorBhutta, Adnan
dc.contributor.authorAyares, David
dc.contributor.authorKaczorowski, David J
dc.contributor.authorMohiuddin, Muhammad M
dc.date.accessioned2021-06-30T15:14:07Z
dc.date.available2021-06-30T15:14:07Z
dc.date.issued2021-06-09
dc.identifier.urihttp://hdl.handle.net/10713/16110
dc.description.abstractBackground: Perioperative cardiac xenograft dysfunction (PCXD) describes a rapidly developing loss of cardiac function after xenotransplantation. PCXD occurs despite genetic modifications to increase compatibility of the heart. We report on the incidence of PCXD using static preservation in ice slush following crystalloid or blood-based cardioplegia versus continuous cold perfusion with XVIVO© heart solution (XHS) based cardioplegia. Methods: Baboons were weight matched to genetically engineered swine heart donors. Cardioplegia volume was 30 cc/kg by donor weight, with del Nido cardioplegia and the addition of 25% by volume of donor whole blood. Continuous perfusion was performed using an XVIVO © Perfusion system with XHS to which baboon RBCs were added. Results: PCXD was observed in 5/8 that were preserved with crystalloid cardioplegia followed by traditional cold, static storage on ice. By comparison, when blood cardioplegia was used followed by cold, static storage, PCXD occurred in 1/3 hearts and only in 1/5 hearts that were induced with XHS blood cardioplegia followed by continuous perfusion. Survival averaged 17 hours in those with traditional preservation and storage, followed by 11.47 days and 15.03 days using blood cardioplegia and XHS+continuous preservation, respectively. Traditional preservation resulted in more inotropic support and higher average peak serum lactate 14.3±1.7 mmol/L compared to blood cardioplegia 3.6±3.0 mmol/L and continuous perfusion 3.5±1.5 mmol/L. Conclusion: Blood cardioplegia induction, alone or followed by XHS perfusion storage, reduced the incidence of PCXD and improved graft function and survival, relative to traditional crystalloid cardioplegia-slush storage alone.en_US
dc.description.urihttps://doi.org/10.3389/fimmu.2021.667093en_US
dc.language.isoenen_US
dc.publisherFrontiers Media S.A.en_US
dc.relation.ispartofFrontiers in Immunologyen_US
dc.rightsCopyright © 2021 Goerlich, Griffith, Singh, Abdullah, Singireddy, Kolesnik, Lewis, Sentz, Tatarov, Hershfeld, Zhang, Strauss, Odonkor, Williams, Tabatabai, Bhutta, Ayares, Kaczorowski and Mohiuddin.en_US
dc.subjectcardiac preservationen_US
dc.subjectcardiac xenotransplantationen_US
dc.subjectgraft dysfunctionen_US
dc.subjectheart failureen_US
dc.subjectheart transplanten_US
dc.subjectventricular assist device (VAD)en_US
dc.subjectxenotransplantationen_US
dc.titleBlood Cardioplegia Induction, Perfusion Storage and Graft Dysfunction in Cardiac Xenotransplantationen_US
dc.typeArticleen_US
dc.identifier.doi10.3389/fimmu.2021.667093
dc.identifier.pmid34177906
dc.source.volume12
dc.source.beginpage667093
dc.source.endpage
dc.source.countrySwitzerland


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