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dc.contributor.authorCannon, Matthew V
dc.contributor.authorBogale, Haikel N
dc.contributor.authorBhalerao, Devika
dc.contributor.authorKeita, Kalil
dc.contributor.authorCamara, Denka
dc.contributor.authorBarry, Yaya
dc.contributor.authorKeita, Moussa
dc.contributor.authorCoulibaly, Drissa
dc.contributor.authorKone, Abdoulaye K
dc.contributor.authorDoumbo, Ogobara K
dc.contributor.authorThera, Mahamadou A
dc.contributor.authorPlowe, Christopher V
dc.contributor.authorTravassos, Mark A
dc.contributor.authorIrish, Seth R
dc.contributor.authorYeroshefsky, Joshua
dc.contributor.authorDorothy, Jeannine
dc.contributor.authorPrendergast, Brian
dc.contributor.authorSt. Laurent, Brandyceen_US
dc.contributor.authorFritz, Megan Len_US
dc.contributor.authorSerre, Daviden_US
dc.date.accessioned2021-06-24T18:17:49Z
dc.date.available2021-06-24T18:17:49Z
dc.date.issued2021-06-22
dc.identifier.urihttp://hdl.handle.net/10713/16080
dc.description.abstractVector-borne pathogens cause many human infectious diseases and are responsible for high mortality and morbidity throughout the world. They can also cause livestock epidemics with dramatic social and economic consequences. Due to its high costs, vector-borne disease surveillance is often limited to current threats, and the investigation of emerging pathogens typically occurs after the reports of clinical cases. Here, we use high-throughput sequencing to detect and identify a wide range of parasites and viruses carried by mosquitoes from Cambodia, Guinea, Mali and Maryland. We apply this approach to individual Anopheles mosquitoes as well as pools of mosquitoes captured in traps; and compare the outcomes of this assay when applied to DNA or RNA. We identified known human and animal pathogens and mosquito parasites belonging to a wide range of taxa, as well as novel DNA sequences from previously uncharacterized organisms. Our results also revealed that analysis of the content of an entire trap could be an efficient approach to monitor and identify rare vector-borne pathogens in large surveillance studies. Overall, we describe a high-throughput and easy-to-customize assay to screen for a wide-range of pathogens and efficiently complement current vector-borne disease surveillance approaches.en_US
dc.description.urihttps://doi.org/10.1242/bio.058855en_US
dc.language.isoenen_US
dc.publisherThe Company of Biologists Ltd.en_US
dc.relation.ispartofBiology Openen_US
dc.rights© 2021. Published by The Company of Biologists Ltd.en_US
dc.subjectArbovirusen_US
dc.subjectEmerging pathogensen_US
dc.subjectEukaryotic parasitesen_US
dc.subjectSurveillanceen_US
dc.subjectVector-borne pathogensen_US
dc.subjectZoonosisen_US
dc.titleHigh-throughput Detection of Eukaryotic Parasites and Arboviruses in Mosquitoesen_US
dc.typeArticleen_US
dc.identifier.doi10.1242/bio.058855
dc.identifier.pmid34156069
dc.source.countryEngland


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