• Login
    View Item 
    •   UMB Digital Archive
    • School, Graduate
    • Theses and Dissertations All Schools
    • View Item
    •   UMB Digital Archive
    • School, Graduate
    • Theses and Dissertations All Schools
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UMB Digital ArchiveCommunitiesPublication DateAuthorsTitlesSubjectsThis CollectionPublication DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    Display statistics

    Paternal effects of lead in the F(1) and F(2) generations of rats: Hippocampal morphology and embryofetal protein synthesis.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Find Full text
    Author
    Gandley, Robin Elizabeth
    Advisor
    Silbergeld, Ellen K.
    Date
    1993
    Type
    dissertation
    
    Metadata
    Show full item record
    Abstract
    The potential for heritable effects of toxicants on development and reproduction via paternal exposure has not been well studied. This study will examine reproductive outcomes in the F{dollar}\sb0{dollar} lead-treated, and F{dollar}\sb1{dollar} males and females from paternal exposure Sprague-Dawley rats. Outcomes assessed were fertility and litter size in freely breeding pairs and progression of superovulated oocytes to 2-cell embryos. Protein synthesis in 2-cell embryos and hippocampal cultures of post-natal day 1 (PN1) offspring was monitored by {dollar}\sp{lcub}35{rcub}{dollar}S-methionine incorporation and 2-dimensional gel electrophoresis. Reproductive tissue lead levels in the F{dollar}\sb0{dollar} were measured using graphite furnace atomic absorption spectrometry. F{dollar}\sb0{dollar} males with B-Pb levels in the range 29-60 {dollar}\mu{dollar}g/dL showed a reduction in breeding success (measured by breeding pairs producing litters), without a reduction in the mean litter size. Lower dosed animals did not show indications of fertility effects. Male and female offspring (F{dollar}\sb1{dollar}) of the high dose animals (F{dollar}\sb0{dollar}) were assessed by natural breeding, superovulation and hippocampal cultures for indications heritable effects of paternal lead exposure. Reductions in both breeding success and mean litter size were observed in F{dollar}\sb1{dollar} males. Effects in F{dollar}\sb1{dollar} females appear to be in breeding success, but litter size could not be adequately determined due to small sample size. Alterations in protein synthesis in the F{dollar}\sb1{dollar} 2-cell embryos were observed as a dose related increase in protein synthesis of the major protein product which is associated with the initial expression of the embryonic genome. F{dollar}\sb1{dollar} 2-cell embryo results indicate male-mediated effects of lead may be observed immediately following the switch from maternal to embryonic control of protein synthesis. Alterations in protein synthesis in F{dollar}\sb1{dollar} and F{dollar}\sb2{dollar} hippocampal cultures are indications of heritable effects of paternal lead exposure. The paternally mediated effects observed in 2-cell embryos and hippocampal cultures may result from more than one effect of lead. It is possible that effects observed in F{dollar}\sb1{dollar} 2-cell embryos are of a regulatory nature, while F{dollar}\sb2{dollar} hippocampal alterations are genetically linked.
    Description
    University of Maryland, Baltimore. Epidemiology and Preventive Medicine. Ph.D. 1993
    Keyword
    Biology, Genetics
    Health Sciences, Toxicology
    Lead--adverse effects
    Paternal Exposure--adverse effects
    Rats, Sprague-Dawley
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/1604
    Collections
    Theses and Dissertations School of Medicine
    Theses and Dissertations All Schools

    entitlement

     
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Policies | Contact Us | UMB Health Sciences & Human Services Library
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.