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dc.contributor.authorMwaba, John
dc.contributor.authorDebes, Amanda K
dc.contributor.authorMurt, Kelsey N
dc.contributor.authorShea, Patrick
dc.contributor.authorSimuyandi, Michelo
dc.contributor.authorLaban, Natasha
dc.contributor.authorKazimbaya, Katayi
dc.contributor.authorChisenga, Caroline
dc.contributor.authorLi, Shan
dc.contributor.authorAlmeida, Mathieu
dc.contributor.authorMeisel, Jacquelyn S
dc.contributor.authorShibemba, Aaron
dc.contributor.authorKantenga, Timothy
dc.contributor.authorMukonka, Victor
dc.contributor.authorKwenda, Geoffrey
dc.contributor.authorSack, David A
dc.contributor.authorChilengi, Roma
dc.contributor.authorStine, O Colin
dc.date.accessioned2021-06-17T12:54:30Z
dc.date.available2021-06-17T12:54:30Z
dc.date.issued2021-06-14
dc.identifier.urihttp://hdl.handle.net/10713/16035
dc.description.abstractBackground: Cholera has been present and recurring in Zambia since 1977. However, there is a paucity of data on genetic relatedness and diversity of the Vibrio cholerae isolates responsible for these outbreaks. Understanding whether the outbreaks are seeded from existing local isolates or if the outbreaks represent separate transmission events can inform public health decisions. Results: Seventy-two V. cholerae isolates from outbreaks in 2009/2010, 2016, and 2017/2018 in Zambia were characterized using multilocus variable number tandem repeat analysis (MLVA) and whole genome sequencing (WGS). The isolates had eight distinct MLVA genotypes that clustered into three MLVA clonal complexes (CCs). Each CC contained isolates from only one outbreak. The results from WGS revealed both clustered and dispersed single nucleotide variants. The genetic relatedness of isolates based on WGS was consistent with the MLVA, each CC was a distinct genetic lineage and had nearest neighbors from other East African countries. In Lusaka, isolates from the same outbreak were more closely related to themselves and isolates from other countries than to isolates from other outbreaks in other years. Conclusions: Our observations are consistent with i) the presence of random mutation and alternative mechanisms of nucleotide variation, and ii) three separate transmission events of V. cholerae into Lusaka, Zambia. We suggest that locally, case-area targeted invention strategies and regionally, well-coordinated plans be in place to effectively control future cholera outbreaks.en_US
dc.description.urihttps://doi.org/10.1186/s12879-021-06259-5en_US
dc.language.isoenen_US
dc.publisherSpringer Natureen_US
dc.relation.ispartofBMC Infectious Diseasesen_US
dc.subjectMLVAen_US
dc.subjectMolecular characterizationen_US
dc.subjectV. choleraeen_US
dc.subjectWhole genome sequencingen_US
dc.subjectZambiaen_US
dc.titleThree transmission events of Vibrio cholerae O1 into Lusaka, Zambiaen_US
dc.typeArticleen_US
dc.identifier.doi10.1186/s12879-021-06259-5
dc.identifier.pmid34126945
dc.source.volume21
dc.source.issue1
dc.source.beginpage570
dc.source.endpage
dc.source.countryEngland


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