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dc.contributor.authorKuscu, Canan
dc.contributor.authorKiran, Manjari
dc.contributor.authorMohammed, Akram
dc.contributor.authorKuscu, Cem
dc.contributor.authorSatpathy, Sarthak
dc.contributor.authorWolen, Aaron
dc.contributor.authorBardhi, Elissa
dc.contributor.authorBajwa, Amandeep
dc.contributor.authorEason, James D.
dc.contributor.authorMaluf, Daniel
dc.contributor.authorMas, Valeria
dc.contributor.authorAkalin, Enver
dc.date.accessioned2021-06-16T14:10:18Z
dc.date.available2021-06-16T14:10:18Z
dc.date.issued2021-06-09
dc.identifier.urihttp://hdl.handle.net/10713/16025
dc.description.abstractTransplant glomerulopathy develops through multiple mechanisms, including donor-specific antibodies, T cells and innate immunity. This study investigates circulating small RNA profiles in serum samples of kidney transplant recipients with biopsy-proven transplant glomerulopathy. Among total small RNA population, miRNAs were the most abundant species in the serum of kidney transplant patients. In addition, fragments arising from mature tRNA and rRNA were detected. Most of the tRNA fragments were generated from 5′ ends of mature tRNA and mainly from two parental tRNAs: tRNA-Gly and tRNA-Glu. Moreover, transplant patients with transplant glomerulopathy displayed a novel tRNA fragments signature. Gene expression analysis from al-lograft tissues demonstrated changes in canonical pathways related to immune activation such as iCos-iCosL signaling pathway in T helper cells, Th1 and Th2 activation pathway, and dendritic cell maturation. mRNA targets of down-regulated miRNAs such as miR-1224-5p, miR-4508, miR-320, miR-378a from serum were globally upregulated in tissue. Integration of serum miRNA profiles with tissue gene expression showed that changes in serum miRNAs support the role of T-cell mediated mechanisms in ongoing allograft injury. © 2021 by the authors.en_US
dc.description.sponsorshipNational Institute of Diabetes and Digestive and Kidney Diseasesen_US
dc.description.urihttps://doi.org/10.3390/ijms22126218en_US
dc.language.isoenen_US
dc.publisherMDPI AGen_US
dc.relation.ispartofInternational Journal of Molecular Sciencesen_US
dc.subjectCirculating small non-coding RNAsen_US
dc.subjectMiRNAsen_US
dc.subjectNon-invasive biomarkeren_US
dc.subjectRNA-seqen_US
dc.subjectTransplant glomerulopathyen_US
dc.subjectTRNA fragmentsen_US
dc.titleIntegrative analyses of circulating small rnas and kidney graft transcriptome in transplant glomerulopathyen_US
dc.typeArticleen_US
dc.identifier.doi10.3390/ijms22126218
dc.source.volume22
dc.source.issue12


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