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dc.contributor.authorRoghmann, Mary-Claire
dc.contributor.authorLydecker, Alison D
dc.contributor.authorShardell, Michelle
dc.contributor.authorDeBoy, Robert T
dc.contributor.authorJohnson, J Kristie
dc.contributor.authorZhao, LiCheng
dc.contributor.authorHittle, Lauren L
dc.contributor.authorMongodin, Emmanuel F
dc.date.accessioned2021-06-11T12:46:12Z
dc.date.available2021-06-11T12:46:12Z
dc.date.issued2021-06-08
dc.identifier.urihttp://hdl.handle.net/10713/15987
dc.description.abstractObjective: To characterize the microbial communities of the anterior nares (nose) and posterior pharynx (throat) of adults dwelling in the community and in nursing homes before and after treatment with intranasal mupirocin. Methods: Staphylococcus aureus-colonized adults were recruited from the community (n = 25) and from nursing homes (n = 7). S. aureus colonization was confirmed using cultures. Participants had specimens taken from nose and throat for S. aureus quantitation using quantitative PCR for the nuc gene and bacterial profiling using 16S rRNA gene sequencing over 12 weeks. After two baseline study visits 4 weeks apart, participants received intranasal mupirocin for 5 days with 3 further visits over a 8 week follow-up period. Results: We found a decrease in the absolute abundance of S. aureus in the nose for 8 weeks after mupirocin (1693 vs 141 fg/ul, p = 0.047). Mupirocin caused a statistically significant disruption in bacterial communities of the nose and throat after 1 week, which was no longer detected after 8 weeks. Bacterial community profiling demonstrated that there was a decrease in the relative abundance of S. aureus (8% vs 0.3%, p<0.01) 8 weeks after mupirocin and a transient decrease in the relative abundance of Staphylococcus epidermidis in the nose (21% vs 5%, p<0.01) 1 week after mupirocin. Conclusions: Decolonization with mupirocin leads to a sustained effect on absolute and relative abundance of S. aureus but not for other bacteria in the nose. This demonstrates that a short course of mupirocin selectively decreases S. aureus in the nose for up to 8 weeks.en_US
dc.description.urihttps://doi.org/10.1371/journal.pone.0252004en_US
dc.language.isoenen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.ispartofPLoS ONEen_US
dc.subject.meshMupirocin--therapeutic useen_US
dc.subject.meshNasopharynxen_US
dc.subject.meshNursing Homesen_US
dc.subject.meshStaphylococcus aureusen_US
dc.titleEffect of mupirocin for Staphylococcus aureus decolonization on the microbiome of the nose and throat in community and nursing home dwelling adultsen_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pone.0252004
dc.identifier.pmid34101737
dc.source.volume16
dc.source.issue6
dc.source.beginpagee0252004
dc.source.endpage
dc.source.countryUnited States


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