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dc.contributor.authorRihtman, Branko
dc.contributor.authorPuxty, Richard J
dc.contributor.authorHapeshi, Alexia
dc.contributor.authorLee, Yan-Jiun
dc.contributor.authorZhan, Yuanchao
dc.contributor.authorMichniewski, Slawomir
dc.contributor.authorWaterfield, Nicholas R
dc.contributor.authorChen, Feng
dc.contributor.authorWeigele, Peter
dc.contributor.authorMillard, Andrew D
dc.contributor.authorScanlan, David J
dc.contributor.authorChen, Yin
dc.date.accessioned2021-06-10T12:53:24Z
dc.date.available2021-06-10T12:53:24Z
dc.date.issued2021-05-24
dc.identifier.urihttp://hdl.handle.net/10713/15978
dc.description.abstractMarine bacterial viruses (bacteriophages) are abundant biological entities that are vital for shaping microbial diversity, impacting marine ecosystem function, and driving host evolution.1-3 The marine roseobacter clade (MRC) is a ubiquitous group of heterotrophic bacteria4,5 that are important in the elemental cycling of various nitrogen, sulfur, carbon, and phosphorus compounds.6-10 Bacteriophages infecting MRC (roseophages) have thus attracted much attention and more than 30 roseophages have been isolated,11-13 the majority of which belong to the N4-like group (Podoviridae family) or the Chi-like group (Siphoviridae family), although ssDNA-containing roseophages are also known.14 In our attempts to isolate lytic roseophages, we obtained two new phages (DSS3_VP1 and DSS3_PM1) infecting the model MRC strain Ruegeria pomeroyi DSS-3. Here, we show that not only do these phages have unusual substitution of deoxythymidine with deoxyuridine (dU) in their DNA, but they are also phylogenetically distinct from any currently known double-stranded DNA bacteriophages, supporting the establishment of a novel family ("Naomiviridae"). These dU-containing phages possess DNA that is resistant to the commonly used library preparation method for metagenome sequencing, which may have caused significant underestimation of their presence in the environment. Nevertheless, our analysis of Tara Ocean metagenome datasets suggests that these unusual bacteriophages are of global importance and more diverse than other well-known bacteriophages, e.g., the Podoviridae in the oceans, pointing to an overlooked role for these novel phages in the environment.en_US
dc.description.urihttps://doi.org/10.1016/j.cub.2021.05.014en_US
dc.language.isoenen_US
dc.publisherCell Pressen_US
dc.relation.ispartofCurrent biology : CBen_US
dc.rightsCopyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.en_US
dc.subjectNaomiviridaeen_US
dc.subjectNoahvirusen_US
dc.subjectdeoxyuridineen_US
dc.subjectmarine roseobactersen_US
dc.subjectnoncanonical DNAen_US
dc.subjectroseophagesen_US
dc.titleA new family of globally distributed lytic roseophages with unusual deoxythymidine to deoxyuridine substitutionen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.cub.2021.05.014
dc.identifier.pmid34033748
dc.source.countryEngland


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