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dc.contributor.authorJiang, Lingling
dc.contributor.authorQi, Yan
dc.contributor.authorKong, Xianghan
dc.contributor.authorWang, Runnan
dc.contributor.authorQi, Jianfei
dc.contributor.authorLin, Francis
dc.contributor.authorCui, Xueling
dc.contributor.authorLiu, Zhonghui
dc.date.accessioned2021-06-09T18:24:29Z
dc.date.available2021-06-09T18:24:29Z
dc.date.issued2021-05-21
dc.identifier.urihttp://hdl.handle.net/10713/15969
dc.description.abstractActivin A, a member of the transforming growth factor-beta (TGF-β) superfamily, contributes to tissue healing and fibrosis. As the innate tissue cells, fibroblasts also play an important role in wound healing and fibrosis. Herein, this study was aimed to investigate how activin A exhibited regulatory effects on adhesion and migration of fibroblasts. We found that activin A induced the migration of fibroblast cell line L929 cells in transwell chamber and microfluidic device. Activin A also promoted L929 cells adhesion, but did not affect L929 cells viability or proliferation. In addition, activin A induced α-SMA expression and TGF-β1 release, which were factors closely related to tissue fibrosis, but had no effect on IL-6 production, a pro-inflammatory cytokine. Furthermore, activin A elevated calcium levels in L929 cells and increased p-ERK protein levels. Activin A-induced migration of L929 cells was attenuated by ERK inhibitor FR180204. To conclude, these data indicated that activin A as a novel chemokine induced the chemotactic migration of L929 cells via ERK signaling and possessed the pro-fibrosis role. These findings provide a new insight into understanding of activin A in tissue fibrosis.en_US
dc.description.urihttps://doi.org/10.3389/fcell.2021.660316en_US
dc.language.isoenen_US
dc.publisherFrontiers Media S.A.en_US
dc.relation.ispartofFrontiers in Cell and Developmental Biologyen_US
dc.rightsCopyright © 2021 Jiang, Qi, Kong, Wang, Qi, Lin, Cui and Liu.en_US
dc.subjectERKen_US
dc.subjectactivin Aen_US
dc.subjectfibroblastsen_US
dc.subjectmicrofluidic deviceen_US
dc.subjectmigrationen_US
dc.titleActivin A as a Novel Chemokine Induces Migration of L929 Fibroblasts by ERK Signaling in Microfluidic Devicesen_US
dc.typeArticleen_US
dc.identifier.doi10.3389/fcell.2021.660316
dc.identifier.pmid34095123
dc.source.volume9
dc.source.beginpage660316
dc.source.endpage
dc.source.countrySwitzerland


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