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    Activin A as a Novel Chemokine Induces Migration of L929 Fibroblasts by ERK Signaling in Microfluidic Devices

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    Author
    Jiang, Lingling
    Qi, Yan
    Kong, Xianghan
    Wang, Runnan
    Qi, Jianfei
    Lin, Francis
    Cui, Xueling
    Liu, Zhonghui
    Date
    2021-05-21
    Journal
    Frontiers in Cell and Developmental Biology
    Publisher
    Frontiers Media S.A.
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.3389/fcell.2021.660316
    Abstract
    Activin A, a member of the transforming growth factor-beta (TGF-β) superfamily, contributes to tissue healing and fibrosis. As the innate tissue cells, fibroblasts also play an important role in wound healing and fibrosis. Herein, this study was aimed to investigate how activin A exhibited regulatory effects on adhesion and migration of fibroblasts. We found that activin A induced the migration of fibroblast cell line L929 cells in transwell chamber and microfluidic device. Activin A also promoted L929 cells adhesion, but did not affect L929 cells viability or proliferation. In addition, activin A induced α-SMA expression and TGF-β1 release, which were factors closely related to tissue fibrosis, but had no effect on IL-6 production, a pro-inflammatory cytokine. Furthermore, activin A elevated calcium levels in L929 cells and increased p-ERK protein levels. Activin A-induced migration of L929 cells was attenuated by ERK inhibitor FR180204. To conclude, these data indicated that activin A as a novel chemokine induced the chemotactic migration of L929 cells via ERK signaling and possessed the pro-fibrosis role. These findings provide a new insight into understanding of activin A in tissue fibrosis.
    Rights/Terms
    Copyright © 2021 Jiang, Qi, Kong, Wang, Qi, Lin, Cui and Liu.
    Keyword
    ERK
    activin A
    fibroblasts
    microfluidic device
    migration
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/15969
    ae974a485f413a2113503eed53cd6c53
    10.3389/fcell.2021.660316
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