Enhancing Metabolic Imaging of Energy Metabolism in Traumatic Brain Injury Using Hyperpolarized [1-C]Pyruvate and Dichloroacetate
AuthorDeVience, Stephen J
Proctor, Julie L
Medina, Juliana A
Melhem, Elias R
Gullapalli, Rao P
MetadataShow full item record
AbstractHyperpolarized magnetic resonance spectroscopic imaging (MRSI) of [1-13C]pyruvate metabolism has previously been used to assess the effects of traumatic brain injury (TBI) in rats. Here, we show that MRSI can be used in conjunction with dichloroacetate to measure the phosphorylation state of pyruvate dehydrogenase (PDH) following mild-to-moderate TBI, and that measurements can be repeated in a longitudinal study to monitor the course of injury progression and recovery. We found that the level of 13C-bicarbonate and the bicarbonate-to-lactate ratio decreased on the injured side of the brain four hours after injury and continued to decrease through day 7. Levels recovered to normal by day 28. Measurements following dichloroacetate administration showed that PDH was inhibited equally by PDH kinase (PDK) on both sides of the brain. Therefore, the decrease in aerobic metabolism is not due to inhibition by PDK.
Keywordcontrolled cortical impact
hyperpolarized metabolic imaging
magnetic resonance spectroscopic imaging
traumatic brain injury
Identifier to cite or link to this itemhttp://hdl.handle.net/10713/15961
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