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dc.contributor.authorAhn, Danielle
dc.contributor.authorBhushan, Gitanjali
dc.contributor.authorMcConville, Thomas H.
dc.contributor.authorAnnavajhala, Medini K.
dc.contributor.authorSoni, Rajesh Kumar
dc.contributor.authorWong Fok Lung, Tania
dc.contributor.authorHofstaedter, Casey E.
dc.contributor.authorShah, Shivang S.
dc.contributor.authorChong, Alexander M.
dc.contributor.authorCastano, Victor G.
dc.contributor.authorErnst, Robert K.
dc.contributor.authorUhlemann, Anne Catrin
dc.contributor.authorPrince, Alice
dc.date.accessioned2021-06-09T15:22:19Z
dc.date.available2021-06-09T15:22:19Z
dc.date.issued2021-06-01
dc.identifier.urihttp://hdl.handle.net/10713/15955
dc.description.abstractKlebsiella pneumoniae ST258 is a human pathogen associated with poor outcomes worldwide. We identify a member of the acyltransferase superfamily 3 (atf3), enriched within the ST258 clade, that provides a major competitive advantage for the proliferation of these organisms in vivo. Comparison of a wild-type ST258 strain (KP35) and a Δatf3 isogenic mutant generated by CRISPR-Cas9 targeting reveals greater NADH:ubiquinone oxidoreductase transcription and ATP generation, fueled by increased glycolysis. The acquisition of atf3 induces changes in the bacterial acetylome, promoting lysine acetylation of multiple proteins involved in central metabolism, specifically Zwf (glucose-6 phosphate dehydrogenase). The atf3-mediated metabolic boost leads to greater consumption of glucose in the host airway and increased bacterial burden in the lung, independent of cytokine levels and immune cell recruitment. Acquisition of this acyltransferase enhances fitness of a K. pneumoniae ST258 isolate and may contribute to the success of this clonal complex as a healthcare-associated pathogen. © 2021 The Authorsen_US
dc.description.urihttps://doi.org/10.1016/j.celrep.2021.109196en_US
dc.language.isoenen_US
dc.publisherElsevier B.V.en_US
dc.relation.ispartofCell Reportsen_US
dc.subjectacetylationen_US
dc.subjectbacterial pneumoniaen_US
dc.subjectglycolysisen_US
dc.subjectKlebsiella pneumoniaeen_US
dc.subjectmetabolismen_US
dc.subjectsequence type 258en_US
dc.titleAn acquired acyltransferase promotes Klebsiella pneumoniae ST258 respiratory infectionen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.celrep.2021.109196
dc.source.volume35
dc.source.issue9


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