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    Minimum information guideline for spectrophotometric and fluorometric methods to assess biofilm formation in microplates

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    Author
    Allkja, Jontana
    Bjarnsholt, Thomas
    Coenye, Tom
    Cos, Paul
    Fallarero, Adyary
    Harrison, Joe J
    Lopes, Susana P
    Oliver, Antonio
    Pereira, Maria Olivia
    Ramage, Gordon
    Shirtliff, Mark E
    Stoodley, Paul
    Webb, Jeremy S
    Zaat, Sebastian A J
    Goeres, Darla M
    Azevedo, Nuno Filipe
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    Date
    2019-11-19
    Journal
    Biofilm
    Publisher
    Elsevier B.V.
    Type
    Article
    
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    See at
    https://doi.org/10.1016/j.bioflm.2019.100010
    Abstract
    The lack of reproducibility of published studies is one of the major issues facing the scientific community, and the field of biofilm microbiology has been no exception. One effective strategy against this multifaceted problem is the use of minimum information guidelines. This strategy provides a guide for authors and reviewers on the necessary information that a manuscript should include for the experiments in a study to be clearly interpreted and independently reproduced. As a result of several discussions between international groups working in the area of biofilms, we present a guideline for the spectrophotometric and fluorometric assessment of biofilm formation in microplates. This guideline has been divided into 5 main sections, each presenting a comprehensive set of recommendations. The intention of the minimum information guideline is to improve the quality of scientific communication that will augment interlaboratory reproducibility in biofilm microplate assays. © 2019 The Author(s)
    Rights/Terms
    © 2019 The Author(s).
    Keyword
    Biofilm
    Fluorometry
    Guidelines
    Microplate
    Reproducibility
    Spectrophotometry
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/15925
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.bioflm.2019.100010
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