Show simple item record

dc.contributor.authorGeorgiadis, Marios
dc.contributor.authorSchroeter, Aileen
dc.contributor.authorGao, Zirui
dc.contributor.authorGuizar-Sicairos, Manuel
dc.contributor.authorLiebi, Marianne
dc.contributor.authorLeuze, Christoph
dc.contributor.authorMcNab, Jennifer A
dc.contributor.authorBalolia, Aleezah
dc.contributor.authorVeraart, Jelle
dc.contributor.authorAdes-Aron, Benjamin
dc.contributor.authorKim, Sunglyoung
dc.contributor.authorShepherd, Timothy
dc.contributor.authorLee, Choong H
dc.contributor.authorWalczak, Piotr
dc.contributor.authorChodankar, Shirish
dc.contributor.authorDiGiacomo, Phillip
dc.contributor.authorDavid, Gergely
dc.contributor.authorAugath, Mark
dc.contributor.authorZerbi, Valerio
dc.contributor.authorSommer, Stefan
dc.contributor.authorRajkovic, Ivan
dc.contributor.authorWeiss, Thomas
dc.contributor.authorBunk, Oliver
dc.contributor.authorYang, Lin
dc.contributor.authorZhang, Jiangyang
dc.contributor.authorNovikov, Dmitry S
dc.contributor.authorZeineh, Michael
dc.contributor.authorFieremans, Els
dc.contributor.authorRudin, Markus
dc.date.accessioned2021-05-28T17:15:30Z
dc.date.available2021-05-28T17:15:30Z
dc.date.issued2021-05-19
dc.identifier.urihttp://hdl.handle.net/10713/15845
dc.description.abstractMyelin insulates neuronal axons and enables fast signal transmission, constituting a key component of brain development, aging and disease. Yet, myelin-specific imaging of macroscopic samples remains a challenge. Here, we exploit myelin's nanostructural periodicity, and use small-angle X-ray scattering tensor tomography (SAXS-TT) to simultaneously quantify myelin levels, nanostructural integrity and axon orientations in nervous tissue. Proof-of-principle is demonstrated in whole mouse brain, mouse spinal cord and human white and gray matter samples. Outcomes are validated by 2D/3D histology and compared to MRI measurements sensitive to myelin and axon orientations. Specificity to nanostructure is exemplified by concomitantly imaging different myelin types with distinct periodicities. Finally, we illustrate the method's sensitivity towards myelin-related diseases by quantifying myelin alterations in dysmyelinated mouse brain. This non-destructive, stain-free molecular imaging approach enables quantitative studies of myelination within and across samples during development, aging, disease and treatment, and is applicable to other ordered biomolecules or nanostructures.en_US
dc.description.urihttps://doi.org/10.1038/s41467-021-22719-7en_US
dc.language.isoenen_US
dc.publisherSpringer Natureen_US
dc.relation.ispartofNature Communicationsen_US
dc.subjectsmall-angle X-ray scattering tensor tomography (SAXS-TT)en_US
dc.subject.meshMyelin Sheathen_US
dc.subject.meshNerve Fibers, Myelinateden_US
dc.subject.meshNerve Tissueen_US
dc.titleNanostructure-specific X-ray tomography reveals myelin levels, integrity and axon orientations in mouse and human nervous tissueen_US
dc.typeArticleen_US
dc.identifier.doi10.1038/s41467-021-22719-7
dc.identifier.pmid34011929
dc.source.volume12
dc.source.issue1
dc.source.beginpage2941
dc.source.endpage
dc.source.countryUnited States
dc.source.countryEngland


This item appears in the following Collection(s)

Show simple item record