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dc.contributor.authorAdediran, Timileyin
dc.date.accessioned2021-05-26T17:46:51Z
dc.date.available2021-05-26T17:46:51Z
dc.date.issued2021
dc.identifier.urihttp://hdl.handle.net/10713/15794
dc.descriptionEpidemiology and Preventive Medicine
dc.descriptionUniversity of Maryland, Baltimore
dc.descriptionPh.D.
dc.description.abstractBackground: Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterial pathogen that leads to an increase in morbidity and mortality. To decrease the spread of MRSA, there is a need to elucidate factors that lead to patient-to-patient transmission in critical care settings. Objectives: Aim 1: To understand if patient-to-patient transmission via HCP mediator differs between high-risk activities by determining the odds of MRSA contamination of the patient from the gown and gloves of the health care personnel (HCP). Aim 2: To determine if isolates found on the gown and gloves of HCP are similar to patient isolates after performing an HCP-patient interaction, using comparative genomic techniques. Methods: Aim 1: This was an observational study of MRSA-positive patients and the HCP who cared for them. We conducted a simulation study of patient-to-patient transmission of MRSA from a HCP vector to a manikin (proxy for the subsequent patient). Using a generalized linear mixed model, we determined the odds of manikin contamination after performing HCP-patient interactions. Aim 2: We selected 95 patient MRSA isolates and their co-isolated HCP gown or glove MRSA isolates using a stratified sampling method. Comparative genomics analyses such as phylogenetic analysis, spa-typing, multi-locus sequence typing (MLST), large-scale blast score ratio (LSBSR), and single nucleotide variant (SNV) analysis were used to achieve this aim’s objective. Results: Aim 1: We observed 103 HCP-patient interactions with 65 MRSA-positive patients and found that subsequent transmission of MRSA from HCP gown and gloves to the manikin proxy occurred 10.7% of the time. There was no association between high-contact patient care activities and MRSA contamination of the manikin following patient care activity (p-value=0.1). Aim 2: Using multiple typing methods, we found that the majority of our isolates were genetically similar. The phylogenetic analysis revealed that 85.2% of paired isolates were similar, and the spa-typing and the LSBSR found that more than 75% of our paired isolates were concordant. However, SNV and MLST identified more than 40% of our paired isolates as discordant. Conclusion: The studies conducted demonstrated patient-to-patient transmission of MRSA via HCP vector, indicating the importance of contact precautions and infection control practices.
dc.subjecthealthcare workers
dc.subjectMRSAen_US
dc.subjectpatient to patient transmissionen_US
dc.subject.meshGenomicsen_US
dc.subject.meshHealth Personnelen_US
dc.subject.meshInfectious Disease Transmission, Patient-to-Professionalen_US
dc.subject.meshInfectious Disease Transmission, Professional-to-Patienten_US
dc.subject.meshMethicillin-Resistant Staphylococcus aureusen_US
dc.titleThe epidemiology of patient to patient transmission of MRSA among critical care patients
dc.typedissertationen_US
dc.date.updated2021-05-21T13:03:21Z
dc.language.rfc3066en
dc.contributor.advisorHarris, Anthony D.
dc.contributor.advisorThom, Kerri
refterms.dateFOA2021-05-26T17:46:52Z


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