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dc.contributor.authorJiang, Bin
dc.contributor.authorLi, Weijie
dc.contributor.authorStewart, Samantha
dc.contributor.authorOu, Wenquan
dc.contributor.authorLiu, Baolin
dc.contributor.authorComizzoli, Pierre
dc.contributor.authorHe, Xiaoming
dc.date.accessioned2021-05-19T20:19:59Z
dc.date.available2021-05-19T20:19:59Z
dc.date.issued2021-04-30
dc.identifier.urihttp://hdl.handle.net/10713/15696
dc.description.sponsorshipHuman induced pluripotent stem cells (hiPSCs) possess tremendous potential for tissue regeneration and banking hiPSCs by cryopreservation for their ready availability is crucial to their widespread use. However, contemporary methods for hiPSC cryopreservation are associated with both limited cell survival and high concentration of toxic cryoprotectants and/or serum. The latter may cause spontaneous differentiation and/or introduce xenogeneic factors, which may compromise the quality of hiPSCs. Here, sand from nature is discovered to be capable of seeding ice above -10 °C, which enables cryopreservation of hiPSCs with no serum, much-reduced cryoprotectant, and high cell survival. Furthermore, the cryopreserved hiPSCs retain high pluripotency and functions judged by their pluripotency marker expression, cell cycle analysis, and capability of differentiation into the three germ layers. This unique sand-mediated cryopreservation method may greatly facilitate the convenient and ready availability of high-quality hiPSCs and probably many other types of cells/tissues for the emerging cell-based translational medicine.en_US
dc.description.urihttps://doi.org/10.1016/j.bioactmat.2021.04.025en_US
dc.language.isoenen_US
dc.publisherElsevier Inc.en_US
dc.relation.ispartofBioactive Materialsen_US
dc.rights© 2021 The Authors.en_US
dc.subjectCryopreservationen_US
dc.subjectIce seedingen_US
dc.subjectSanden_US
dc.subjectStem cellen_US
dc.subjectiPSCen_US
dc.titleSand-mediated ice seeding enables serum-free low-cryoprotectant cryopreservation of human induced pluripotent stem cells.en_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.bioactmat.2021.04.025
dc.identifier.pmid33997514
dc.source.volume6
dc.source.issue12
dc.source.beginpage4377
dc.source.endpage4388
dc.source.countryChina


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