• Login
    View Item 
    •   UMB Digital Archive
    • UMB Open Access Articles
    • UMB Open Access Articles
    • View Item
    •   UMB Digital Archive
    • UMB Open Access Articles
    • UMB Open Access Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UMB Digital ArchiveCommunitiesPublication DateAuthorsTitlesSubjectsThis CollectionPublication DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    Display statistics

    Sand-mediated ice seeding enables serum-free low-cryoprotectant cryopreservation of human induced pluripotent stem cells.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Publisher version
    View Source
    Access full-text PDFOpen Access
    View Source
    Check access options
    Check access options
    Author
    Jiang, Bin
    Li, Weijie
    Stewart, Samantha
    Ou, Wenquan
    Liu, Baolin
    Comizzoli, Pierre
    He, Xiaoming
    Date
    2021-04-30
    Journal
    Bioactive Materials
    Publisher
    Elsevier Inc.
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.1016/j.bioactmat.2021.04.025
    Sponsors
    Human induced pluripotent stem cells (hiPSCs) possess tremendous potential for tissue regeneration and banking hiPSCs by cryopreservation for their ready availability is crucial to their widespread use. However, contemporary methods for hiPSC cryopreservation are associated with both limited cell survival and high concentration of toxic cryoprotectants and/or serum. The latter may cause spontaneous differentiation and/or introduce xenogeneic factors, which may compromise the quality of hiPSCs. Here, sand from nature is discovered to be capable of seeding ice above -10 °C, which enables cryopreservation of hiPSCs with no serum, much-reduced cryoprotectant, and high cell survival. Furthermore, the cryopreserved hiPSCs retain high pluripotency and functions judged by their pluripotency marker expression, cell cycle analysis, and capability of differentiation into the three germ layers. This unique sand-mediated cryopreservation method may greatly facilitate the convenient and ready availability of high-quality hiPSCs and probably many other types of cells/tissues for the emerging cell-based translational medicine.
    Rights/Terms
    © 2021 The Authors.
    Keyword
    Cryopreservation
    Ice seeding
    Sand
    Stem cell
    iPSC
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/15696
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.bioactmat.2021.04.025
    Scopus Count
    Collections
    UMB Open Access Articles

    entitlement

    Related articles

    • Freezing Responses in DMSO-Based Cryopreservation of Human iPS Cells: Aggregates Versus Single Cells.
    • Authors: Li R, Yu G, Azarin SM, Hubel A
    • Issue date: 2018 May
    • Cell Banking of hiPSCs: A Practical Guide to Cryopreservation and Quality Control in Basic Research.
    • Authors: Shibamiya A, Schulze E, Krauß D, Augustin C, Reinsch M, Schulze ML, Steuck S, Mearini G, Mannhardt I, Schulze T, Klampe B, Werner T, Saleem U, Knaust A, Laufer SD, Neuber C, Lemme M, Behrens CS, Loos M, Weinberger F, Fuchs S, Eschenhagen T, Hansen A, Ulmer BM
    • Issue date: 2020 Dec
    • A simple and efficient cryopreservation method for feeder-free dissociated human induced pluripotent stem cells and human embryonic stem cells.
    • Authors: Mollamohammadi S, Taei A, Pakzad M, Totonchi M, Seifinejad A, Masoudi N, Baharvand H
    • Issue date: 2009 Oct
    • Predehydration and Ice Seeding in the Presence of Trehalose Enable Cell Cryopreservation.
    • Authors: Huang H, Zhao G, Zhang Y, Xu J, Toth TL, He X
    • Issue date: 2017 Aug 14
    • Cryopreservation of human induced pluripotent stem cells by using a new CryoLogic vitrification method.
    • Authors: Zhang Y, Liu H, Liu Z, Long P, Zhao X, Li Q, Huang Y, Ma Y
    • Issue date: 2021 Feb
    DSpace software (copyright © 2002 - 2022)  DuraSpace
    Quick Guide | Policies | Contact Us | UMB Health Sciences & Human Services Library
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.