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dc.contributor.authorLee, Rachel M
dc.contributor.authorVitolo, Michele I
dc.contributor.authorLosert, Wolfgang
dc.contributor.authorMartin, Stuart S
dc.date.accessioned2021-05-17T13:59:30Z
dc.date.available2021-05-17T13:59:30Z
dc.date.issued2021-05-13
dc.identifier.urihttp://hdl.handle.net/10713/15656
dc.description.abstractRecent evidence suggests that groups of cells are more likely to form clinically dangerous metastatic tumors, emphasizing the importance of understanding mechanisms underlying collective behavior. The emergent collective behavior of migrating cell sheets in vitro has been shown to be disrupted in tumorigenic cells but the connection between this behavior and in vivo tumorigenicity remains unclear. We use particle image velocimetry to measure a multidimensional migration phenotype for genetically defined human breast epithelial cell lines that range in their in vivo behavior from non-tumorigenic to aggressively metastatic. By using cells with controlled mutations, we show that PTEN deletion enhances collective migration, while Ras activation suppresses it, even when combined with PTEN deletion. These opposing effects on collective migration of two mutations that are frequently found in patient tumors could be exploited in the development of novel treatments for metastatic disease. Our methods are based on label-free phase contrast imaging, and thus could easily be applied to patient tumor cells. The short time scales of our approach do not require potentially selective growth, and thus in combination with label-free imaging would allow multidimensional collective migration phenotypes to be utilized in clinical assessments of metastatic potential.en_US
dc.description.urihttps://doi.org/10.1038/s41598-021-89130-6en_US
dc.description.urihttp://www.ncbi.nlm.nih.gov/pmc/articles/pmc8119502/en_US
dc.language.isoenen_US
dc.publisherSpringer Natureen_US
dc.relation.ispartofScientific Reportsen_US
dc.subject.meshCell Movementen_US
dc.subject.meshNeoplasm Metastasisen_US
dc.subject.meshNeoplasms--geneticsen_US
dc.titleDistinct roles of tumor associated mutations in collective cell migrationen_US
dc.typeArticleen_US
dc.identifier.doi10.1038/s41598-021-89130-6
dc.identifier.pmid33986306
dc.source.volume11
dc.source.issue1
dc.source.beginpage10291
dc.source.endpage
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryEngland


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