Show simple item record

dc.contributor.authorO'Hanlon, D Elizabeth
dc.contributor.authorBrown, Sarah E
dc.contributor.authorHe, Xin
dc.contributor.authorStennett, Christina A
dc.contributor.authorRobbins, Sarah J
dc.contributor.authorJohnston, Elizabeth D
dc.contributor.authorWnorowski, Amelia M
dc.contributor.authorMark, Katrina
dc.contributor.authorRavel, Jacques
dc.contributor.authorCone, Richard A
dc.contributor.authorBrotman, Rebecca M
dc.date.accessioned2021-05-05T17:40:14Z
dc.date.available2021-05-05T17:40:14Z
dc.date.issued2021-05-03
dc.identifier.urihttp://hdl.handle.net/10713/15584
dc.description.abstractThe outer layers of the vaginal epithelium (VE) are important because they accumulate glycogen which, under optimal conditions, Lactobacillus spp. consume to grow and acidify the vaginal microenvironment with lactic acid. We hypothesized that exposure to lubricant, for example in the conduct of a transvaginal ultrasound (TVUS), may contribute to the shedding of mature epithelial cells, exposing immature cells. Cervicovaginal fluid (CVF) was sampled at four time points by menstrual cup (Softdisc™) from 50 women referred for TVUS, during which a controlled volume of lubricant was applied to the TVUS wand. Samples were collected (1) immediately before TVUS and (2) 6-12 hours, (3) within one week, and (4) two weeks after TVUS. Clinical vaginal lubricants are similar to commercial lubricants, and often have a high osmolality or pH, and contain bactericides such as methylparaben and propylparaben. The number and maturity of epithelial cells in each CVF sample were measured by quantitative and differential fluorimetry (maturity index, MI). Comparisons of cell-counts and maturity were made by paired Wilcoxon signed-rank tests. Among women with a high pre-TVUS MI (> 3), there was a decrease in median cell-count and mean MI in the sample collected 6-12 hours after TVUS (p<0.001, n = 26 and p < 0.001, n = 26, respectively). For these women, cell-count and MI remained lower in the sample collected within the subsequent week (p<0.001, n = 29 and p<0.01, n = 29, respectively), and MI remained lower in the sample collected within two weeks of TVUS (p<0.01, n = 25), compared to the pre-TVUS sample. Among participants with a low pre-TVUS MI (< 3), cell-count was higher in the sample collected within two weeks of TVUS compared to the pre-TVUS sample (p = 0.03, n = 15), but no significant changes in MI were observed. Results were similar when restricted to reproductive-age women. This preliminary data indicates hypertonic vaginal lubricants may increase vaginal epithelial cell shedding.en_US
dc.description.urihttps://doi.org/10.1371/journal.pone.0250153en_US
dc.language.isoenen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.ispartofPLoS ONEen_US
dc.subjectvaginal epithelial cell sheddingen_US
dc.subject.meshLubricants--adverse effectsen_US
dc.subject.meshVaginaen_US
dc.titleObservational cohort study of the effect of a single lubricant exposure during transvaginal ultrasound on cell-shedding from the vaginal epitheliumen_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pone.0250153
dc.identifier.pmid33939727
dc.source.volume16
dc.source.issue5
dc.source.beginpagee0250153
dc.source.endpage
dc.source.countryUnited States


This item appears in the following Collection(s)

Show simple item record