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    A pilot study of an anti-endotoxin Ig-enriched bovine colostrum to prevent experimental sepsis

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    Author
    Cross, Alan S
    Opal, Steven M
    Palardy, John E
    Shridhar, Surekha
    Baliban, Scott M
    Scott, Alison J
    Chahin, Abdullah B
    Ernst, Robert K
    Date
    2021-04-16
    Journal
    Innate Immunity
    Publisher
    SAGE Publications Inc.
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.1177/17534259211007538
    Abstract
    Despite the dramatic increase in antimicrobial resistance, there is a dearth of antibiotics in development and few pharmaceutical companies working in the field. Further, any new antibiotics are likely to have a short shelf life. Ab-based interventions offer alternatives that are not likely to be circumvented by the widely prevalent antibiotic resistance genes. Bovine colostrum (BC)-the first milk after parturition, rich in nutrients and immune components-promotes gut integrity and modulates the gut microbiome. We developed a hyperimmune BC (HBC) enriched in Abs to a highly conserved LOS core region of Gram-negative bacteria by immunizing pregnant cows with a vaccine comprised of detoxified LOS from Escherichia coli O111 Rc (J5) mutant non-covalently complexed to group B meningococcal outer membrane protein (J5dLOS/OMP). This vaccine generated robust levels of anti-J5 LOS Ab in the colostrum. When given orally to neutropenic rats challenged orally with Pseudomonas aeruginosa, administration of HBC improved survival compared to non-immune rats, while both BC preparations improved survival compared to PBS controls. Elevated circulating endotoxin levels correlated with mortality. HBC and to a lesser extent non-immune BC reduced bacterial burden from the liver, lung, and spleen. We conclude that HBC and to a lesser extent BC may be effective supplements that improve outcome from lethal gut-derived disseminated infection and may reduce transmission of Gram-negative bacilli from the gastrointestinal tract.
    Keyword
    Antibody
    Pseudomonas aeruginosa
    antimicrobial resistance
    bovine colostrum
    endotoxin
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/15542
    ae974a485f413a2113503eed53cd6c53
    10.1177/17534259211007538
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