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dc.contributor.authorVila, T.
dc.contributor.authorKong, E.F.
dc.contributor.authorMontelongo-Jauregui, D.
dc.contributor.authorVan Dijck, P.en_US
dc.contributor.authorShetty, A.C.en_US
dc.contributor.authorMcCracken, C.en_US
dc.contributor.authorBruno, V.M.en_US
dc.contributor.authorJabra-Rizk, M.A.en_US
dc.date.accessioned2021-04-12T17:10:16Z
dc.date.available2021-04-12T17:10:16Z
dc.date.issued2021-03-08
dc.identifier.urihttp://hdl.handle.net/10713/15266
dc.description.abstractBiofilm-associated polymicrobial infections tend to be challenging to treat. Candida albicans and Staphylococcus aureus are leading pathogens due to their ability to form biofilms on medical devices. However, the therapeutic implications of their interactions in a host is largely unexplored. In this study, we used a mouse subcutaneous catheter model for in vivo-grown polymicrobial biofilms to validate our in vitro findings on C. albicans-mediated enhanced S. aureus tolerance to vancomycin in vivo. Comparative assessment of S. aureus recovery from catheters with single- or mixed-species infection demonstrated failure of vancomycin against S. aureus in mice with co-infected catheters. To provide some mechanistic insights, RNA-seq analysis was performed on catheter biofilms to delineate transcriptional modulations during polymicrobial infections. C. albicans induced the activation of the S. aureus biofilm formation network via down-regulation of the lrg operon, repressor of autolysis, and up-regulation of the ica operon and production of polysaccharide intercellular adhesin (PIA), indicating an increase in eDNA production, and extracellular polysaccharide matrix, respectively. Interestingly, virulence factors important for disseminated infections, and superantigen-like proteins were down-regulated during mixed-species infection, whereas capsular polysaccharide genes were up-regulated, signifying a strategy favoring survival, persistence and host immune evasion. In vitro follow-up experiments using DNA enzymatic digestion, lrg operon mutant strains, and confocal scanning microscopy confirmed the role of C. albicans-mediated enhanced eDNA production in mixed-biofilms on S. aureus tolerance to vancomycin. Combined, these findings provide mechanistic insights into the therapeutic implications of interspecies interactions, underscoring the need for novel strategies to overcome limitations of current therapies. Copyright 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.en_US
dc.description.sponsorshipThe work in this publication was supported by the National Institute of Allergy and Infectious Diseases of the NIH under award numbers R01AI130170 (NIAID) to M.A.J-R and U19AI110820 to V.M.B;National Institutes of Health [R01AI130170];National Institutes of Health [U19AI110820];en_US
dc.description.urihttps://doi.org/10.1080/21505594.2021.1894834en_US
dc.language.isoen_USen_US
dc.publisherBellwether Publishing, Ltd.en_US
dc.relation.ispartofVirulence
dc.subjectCandidaen_US
dc.subjectmixed-biofilmsen_US
dc.subjectpolymicrobial infectionsen_US
dc.subjectStaphylococcusen_US
dc.subjecttranscriptomeen_US
dc.titleTherapeutic implications of C. albicans-S. aureus mixed biofilm in a murine subcutaneous catheter model of polymicrobial infectionen_US
dc.typeArticleen_US
dc.identifier.doi10.1080/21505594.2021.1894834
dc.identifier.pmid33682623


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