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    Calcium-induced troponin flexibility revealed by distance distribution measurements between engineered sites

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    Author
    Zhao, Xinmei
    Advisor
    Collins, John H.
    Date
    1995
    Type
    dissertation
    
    Metadata
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    Abstract
    Calcium regulation of vertebrate striated muscle contraction is initiated by conformational changes in the Ca2+-binding protein troponin C (TnC) and subsequent changes in the interaction of TnC with the inhibitory protein TnI and the tropomyosin-binding protein TnT. We have used the frequency domain method of fluorescence resonance energy transfer to measure TnT-TnC, TnT-TnI and TnI-TnC distances and distributions, in the presence of Ca2+, Mg2+, or EGTA, in TnC-TnI-TnT and TnC-TnI complexes. We reconstituted functional, ternary troponin complexes using the following recombinant subunits whose sequences were based on those of rabbit skeletal muscle: wild-type TnC; {dollar}\rm TnT\sb{lcub}25{rcub},{dollar} a mutant C-terminal 25-kDa fragment of TnT containing a single Trp-212 which was used as the sole donor for fluorescence energy transfer measurements; Trp-less TnI mutants which contained either no Cys or a single Cys at position 9, 96, or 117. The binary Tn complex (TnI-TnC) was reconstituted by using wild-type TnC and mutant TnI which contained no Cys and a single Trp at position 106. Energy acceptor groups were introduced into TnC or TnI by labeling with dansyl aziridine or N-(iodoacetyl)-{dollar}\rm N\sp\prime{dollar}-(1-sulfo-5-naphthyl)ethylenediamine. Our results indicate that the troponin complex is relatively rigid in relaxed muscle, but becomes much more flexible when Ca2+ binds to regulatory sites in TnC. This increased flexibility may be propagated to the whole thin filament, releasing the inhibition of actomyosin ATPase activity and allowing the muscle to contract.
    Description
    University of Maryland, Baltimore. Ph.D. 1995
    Keyword
    Biology, Molecular
    Chemistry, Biochemistry
    Engineering, Biomedical
    Calcium-Binding Proteins
    Muscle, Striated
    Troponin--physiology
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/1524
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    Theses and Dissertations All Schools

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