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dc.contributor.authorSutton, H.J.
dc.contributor.authorAye, R.
dc.contributor.authorIdris, A.H.
dc.contributor.authorVistein, R.
dc.contributor.authorNduati, E.
dc.contributor.authorKai, O.
dc.contributor.authorMwacharo, J.
dc.contributor.authorLi, X.
dc.contributor.authorGao, X.
dc.contributor.authorAndrews, T.D.
dc.contributor.authorKoutsakos, M.
dc.contributor.authorNguyen, T.H.O.
dc.contributor.authorNekrasov, M.
dc.contributor.authorMilburn, P.
dc.contributor.authorEltahla, A.
dc.contributor.authorBerry, A.A.
dc.contributor.authorKC, N.
dc.contributor.authorChakravarty, S.
dc.contributor.authorSim, B.K.L.
dc.contributor.authorWheatley, A.K.
dc.contributor.authorKent, S.J.
dc.contributor.authorHoffman, S.L.
dc.contributor.authorLyke, K.E.
dc.contributor.authorBejon, P.
dc.contributor.authorLuciani, F.
dc.contributor.authorKedzierska, K.
dc.contributor.authorSeder, R.A.
dc.contributor.authorNdungu, F.M.
dc.contributor.authorCockburn, I.A.
dc.date.accessioned2021-04-12T16:48:46Z
dc.date.available2021-04-12T16:48:46Z
dc.date.issued2021-02-09
dc.identifier.urihttp://hdl.handle.net/10713/15226
dc.description.abstractThe diversity of circulating human B cells is unknown. We use single-cell RNA sequencing (RNA-seq) to examine the diversity of both antigen-specific and total B cells in healthy subjects and malaria-exposed individuals. This reveals two B cell lineages: a classical lineage of activated and resting memory B cells and an alternative lineage, which includes previously described atypical B cells. Although atypical B cells have previously been associated with disease states, the alternative lineage is common in healthy controls, as well as malaria-exposed individuals. We further track Plasmodium-specific B cells after malaria vaccination in naive volunteers. We find that alternative lineage cells are primed after the initial immunization and respond to booster doses. However, alternative lineage cells develop an atypical phenotype with repeated boosts. The data highlight that atypical cells are part of a wider alternative lineage of B cells that are a normal component of healthy immune responses. Copyright 2021 The Author(s)Using single-cell RNA sequencing, Sutton et al. show that a population of "atypical" B cells, normally associated with disease, are part of a wider landscape of alternative B cells that participate in normal responses to vaccination. Copyright 2021 The Author(s)en_US
dc.description.urihttps://doi.org/10.1016/j.celrep.2020.108684en_US
dc.language.isoen_USen_US
dc.publisherElsevier B.V.en_US
dc.relation.ispartofCell Reports
dc.subjectalternative B cell lineageen_US
dc.subjectatypical B cellsen_US
dc.subjectB cell memoryen_US
dc.subjectCITE-seqen_US
dc.subjectmalariaen_US
dc.subjectsingle cell RNA-seqen_US
dc.subjectsporozoiteen_US
dc.subjectvaccinationen_US
dc.titleAtypical B cells are part of an alternative lineage of B cells that participates in responses to vaccination and infection in humansen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.celrep.2020.108684
dc.identifier.pmid33567273


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