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    Atypical B cells are part of an alternative lineage of B cells that participates in responses to vaccination and infection in humans

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    Author
    Sutton, H.J.
    Aye, R.
    Idris, A.H.
    Vistein, R.
    Nduati, E.
    Kai, O.
    Mwacharo, J.
    Li, X.
    Gao, X.
    Andrews, T.D.
    Koutsakos, M.
    Nguyen, T.H.O.
    Nekrasov, M.
    Milburn, P.
    Eltahla, A.
    Berry, A.A.
    KC, N.
    Chakravarty, S.
    Sim, B.K.L.
    Wheatley, A.K.
    Kent, S.J.
    Hoffman, S.L.
    Lyke, K.E.
    Bejon, P.
    Luciani, F.
    Kedzierska, K.
    Seder, R.A.
    Ndungu, F.M.
    Cockburn, I.A.
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    Date
    2021-02-09
    Journal
    Cell Reports
    Publisher
    Elsevier B.V.
    Type
    Article
    
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    Show full item record
    See at
    https://doi.org/10.1016/j.celrep.2020.108684
    Abstract
    The diversity of circulating human B cells is unknown. We use single-cell RNA sequencing (RNA-seq) to examine the diversity of both antigen-specific and total B cells in healthy subjects and malaria-exposed individuals. This reveals two B cell lineages: a classical lineage of activated and resting memory B cells and an alternative lineage, which includes previously described atypical B cells. Although atypical B cells have previously been associated with disease states, the alternative lineage is common in healthy controls, as well as malaria-exposed individuals. We further track Plasmodium-specific B cells after malaria vaccination in naive volunteers. We find that alternative lineage cells are primed after the initial immunization and respond to booster doses. However, alternative lineage cells develop an atypical phenotype with repeated boosts. The data highlight that atypical cells are part of a wider alternative lineage of B cells that are a normal component of healthy immune responses. Copyright 2021 The Author(s)Using single-cell RNA sequencing, Sutton et al. show that a population of "atypical" B cells, normally associated with disease, are part of a wider landscape of alternative B cells that participate in normal responses to vaccination. Copyright 2021 The Author(s)
    Keyword
    alternative B cell lineage
    atypical B cells
    B cell memory
    CITE-seq
    malaria
    single cell RNA-seq
    sporozoite
    vaccination
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/15226
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.celrep.2020.108684
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