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dc.contributor.authorDeng, R.
dc.contributor.authorKao, J.P.Y.
dc.contributor.authorKanold, P.O.
dc.date.accessioned2021-04-12T16:16:40Z
dc.date.available2021-04-12T16:16:40Z
dc.date.issued2021-02-4
dc.identifier.urihttp://hdl.handle.net/10713/15170
dc.description.abstractThe development of GABAergic interneurons is important for the functional maturation of cortical circuits. After migrating into the cortex, GABAergic interneurons start to receive glutamatergic connections from cortical excitatory neurons and thus gradually become integrated into cortical circuits. These glutamatergic connections are mediated by glutamate receptors including AMPA and NMDA receptors and the ratio of AMPA to NMDA receptors decreases during development. Since previous studies have shown that retinal input can regulate the early development of connections along the visual pathway, we investigated if the maturation of glutamatergic inputs to GABAergic interneurons in the visual cortex requires retinal input. We mapped the spatial pattern of glutamatergic connections to layer 4 (L4) GABAergic interneurons in mouse visual cortex at around postnatal day (P) 16 by laser-scanning photostimulation and investigated the effect of binocular enucleations at P1/P2 on these patterns. Gad2-positive interneurons in enucleated animals showed an increased fraction of AMPAR-mediated input from L2/3 and a decreased fraction of input from L5/6. Parvalbumin-expressing (PV) interneurons showed similar changes in relative connectivity. NMDAR-only input was largely unchanged by enucleation. Our results show that retinal input sculpts the integration of interneurons into V1 circuits and suggest that the development of AMPAR- and NMDAR-only connections might be regulated differently. Copyright 2021, The Author(s).en_US
dc.description.sponsorshipSupported by NIH R01DC009607 (POK) and R01GM056481 (JPYK).en_US
dc.description.urihttps://doi.org/10.1038/s41598-021-82679-2en_US
dc.language.isoen_USen_US
dc.publisherNature Researchen_US
dc.relation.ispartofScientific Reports
dc.subjectcortical circuitsen_US
dc.subject.meshNeurogenesisen_US
dc.subject.meshRetinaen_US
dc.subject.meshVisual Cortex--growth & developmenten_US
dc.titleAberrant development of excitatory circuits to inhibitory neurons in the primary visual cortex after neonatal binocular enucleationen_US
dc.typeArticleen_US
dc.identifier.doi10.1038/s41598-021-82679-2
dc.identifier.pmid33542365


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