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dc.contributor.authorSong, J.H.
dc.contributor.authorTieu, A.H.
dc.contributor.authorCheng, Y.
dc.contributor.authorMa, K.
dc.contributor.authorAkshintala, V.S.
dc.contributor.authorSimsek, C.
dc.contributor.authorPrasath, V.
dc.contributor.authorShin, E.J.
dc.contributor.authorNgamruengphong, S.
dc.contributor.authorKhashab, M.A.
dc.contributor.authorAbraham, J.M.
dc.contributor.authorMeltzer, S.J.
dc.date.accessioned2021-04-12T13:11:08Z
dc.date.available2021-04-12T13:11:08Z
dc.date.issued2021-04-03
dc.identifier.urihttp://hdl.handle.net/10713/15146
dc.description.abstractBarrett’s esophagus (BE) is a precursor to esophageal adenocarcinoma (EAC). Recently, long noncoding RNAs (lncRNAs) have been identified as key regulators of biological pathways. However, involvement of lncRNAs in the development of BE and EAC has not been well-studied. The aims of the current study were: (1) to study involvement of the lncRNA, miR205HG, in the development of BE and EAC; (2) to clarify the role of miR205HG in in vitro and in vivo experiments; and (3) to investigate the mechanism of miR205HG involving the Hedgehog (Hh) signaling pathway. These experiments revealed that miR205HG was downregulated in EAC vs. normal esophageal epithelia (NE) as well as in EAC cell lines, and its forced overexpression inhibited EAC cell proliferation and cell cycle progression in vitro. Similarly, overexpression of miR205HG inhibited xenograft tumor growth in mice In vivo. Finally, we show that one mechanism of action of miR205HG involves the Hh signaling pathway: miR205HG and Hh expression levels were inversely correlated in both EAC (r = −0.73) and BE (r = −0.83) tissues, and in vitro studies revealed details of Hh signaling inhibition induced by miR205HG. In conclusion, these findings establish that lncRNA miR205HG functions as a tumor suppressor in the development of BE and EAC, at least in part through its effect on the Hh signaling pathway. Copyright 2021 by the authors.en_US
dc.description.sponsorshipFunding: This work was supported by the National Institutes of Health (Grants CA211457 and DK118250), the Emerson Cancer Research Fund, and a Discovery Award from The Johns Hopkins University School of Medicine.en_US
dc.description.urihttps://doi.org/10.3390/cancers13071707en_US
dc.language.isoen_USen_US
dc.publisherMDPI AGen_US
dc.relation.ispartofCancers
dc.subjectBarrett's esophagusen_US
dc.subjectEsophageal adenocarcinoma (EAC)en_US
dc.subjectHedgehog inhibitoren_US
dc.subjectLong noncoding RNA (lncRNAs)en_US
dc.titleNovel long noncoding rna mir205hg functions as an esophageal tumor-suppressive hedgehog inhibitoren_US
dc.typeArticleen_US
dc.identifier.doi10.3390/cancers13071707


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