Novel Extracellular Electron Transfer Channels in a Gram-Positive Thermophilic Bacterium.
AuthorGavrilov, Sergey N
Zavarzina, Daria G
Elizarov, Ivan M
Tikhonova, Tamara V
Dergousova, Natalia I
Popov, Vladimir O
Lloyd, Jonathan R
El-Naggar, Mohamed Y
Leung, Kar Man
Robb, Frank T
Zakhartsev, Maksim V
Bonch-Osmolovskaya, Elizaveta A
JournalFrontiers in Microbiology
PublisherFrontiers Media S.A.
MetadataShow full item record
AbstractBiogenic transformation of Fe minerals, associated with extracellular electron transfer (EET), allows microorganisms to exploit high-potential refractory electron acceptors for energy generation. EET-capable thermophiles are dominated by hyperthermophilic archaea and Gram-positive bacteria. Information on their EET pathways is sparse. Here, we describe EET channels in the thermophilic Gram-positive bacterium Carboxydothermus ferrireducens that drive exoelectrogenesis and rapid conversion of amorphous mineral ferrihydrite to large magnetite crystals. Microscopic studies indicated biocontrolled formation of unusual formicary-like ultrastructure of the magnetite crystals and revealed active colonization of anodes in bioelectrochemical systems (BESs) by C. ferrireducens. The internal structure of micron-scale biogenic magnetite crystals is reported for the first time. Genome analysis and expression profiling revealed three constitutive c-type multiheme cytochromes involved in electron exchange with ferrihydrite or an anode, sharing insignificant homology with previously described EET-related cytochromes thus representing novel determinants of EET. Our studies identify these cytochromes as extracellular and reveal potentially novel mechanisms of cell-to-mineral interactions in thermal environments.
Rights/TermsCopyright © 2021 Gavrilov, Zavarzina, Elizarov, Tikhonova, Dergousova, Popov, Lloyd, Knight, El-Naggar, Pirbadian, Leung, Robb, Zakhartsev, Bretschger and Bonch-Osmolovskaya.
biogenic magnetite crystals
Identifier to cite or link to this itemhttp://hdl.handle.net/10713/14697
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