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dc.contributor.authorLebov, Jarrett F
dc.contributor.authorBohannan, Brendan J M
dc.date.accessioned2021-02-15T21:31:32Z
dc.date.available2021-02-15T21:31:32Z
dc.date.issued2021-01-20
dc.identifier.urihttp://hdl.handle.net/10713/14693
dc.description.abstractSymbioses between animals and bacteria are ubiquitous. To better understand these relationships, it is essential to unravel how bacteria evolve to colonize hosts. Previously, we serially passaged the free-living bacterium, Shewanella oneidensis, through the digestive tracts of germ-free larval zebrafish (Danio rerio) to uncover the evolutionary changes involved in the initiation of a novel symbiosis with a vertebrate host. After 20 passages, we discovered an adaptive missense mutation in the mshL gene of the msh pilus operon, which improved host colonization, increased swimming motility, and reduced surface adhesion. In the present study, we determined that this mutation was a loss-of-function mutation and found that it improved zebrafish colonization by augmenting S. oneidensis representation in the water column outside larvae through a reduced association with environmental surfaces. Additionally, we found that strains containing the mshL mutation were able to immigrate into host digestive tracts at higher rates per capita. However, mutant and evolved strains exhibited no evidence of a competitive advantage after colonizing hosts. Our results demonstrate that bacterial behaviors outside the host can play a dominant role in facilitating the onset of novel host associations.en_US
dc.description.urihttps://doi.org/10.3390/genes12020127en_US
dc.language.isoenen_US
dc.publisherMDPI AGen_US
dc.relation.ispartofGenesen_US
dc.subjectadaptationen_US
dc.subjectbiofilmsen_US
dc.subjectcolonizationen_US
dc.subjectfitnessen_US
dc.subjecthost–microbeen_US
dc.subjectmicrobial evolutionen_US
dc.subjectmsh pilusen_US
dc.subjectshewanellaen_US
dc.subjectsymbiosisen_US
dc.subjectzebrafishen_US
dc.titleMsh Pilus Mutations Increase the Ability of a Free-Living Bacterium to Colonize a Piscine Host.en_US
dc.typeArticleen_US
dc.identifier.doi10.3390/genes12020127
dc.identifier.pmid33498301
dc.source.volume12
dc.source.issue2
dc.source.countryUnited States
dc.source.countrySwitzerland


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