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dc.contributor.authorLiu, Jie
dc.contributor.authorPholwat, Suporn
dc.contributor.authorZhang, Jixian
dc.contributor.authorTaniuchi, Mami
dc.contributor.authorHaque, Rashidul
dc.contributor.authorAlam, Masud
dc.contributor.authorOchieng, John Benjamin
dc.contributor.authorJones, Jennifer A
dc.contributor.authorPlatts-Mills, James A
dc.contributor.authorTennant, Sharon M
dc.contributor.authorHoupt, Eric
dc.date.accessioned2021-02-12T16:44:42Z
dc.date.available2021-02-12T16:44:42Z
dc.date.issued2021-01-21
dc.identifier.urihttp://hdl.handle.net/10713/14686
dc.description.abstractShigella flexneri is prevalent worldwide and is the most common Shigella species in many countries. At least 19 S. flexneri serotypes exist, and serotype information is important for epidemiologic and vaccine development purposes. We evaluated the performance of real-time PCR assays for O-antigen modification genes to identify the major serotypes on isolates and direct stool samples. The assays were formulated into two multiplex panels: one panel included gtrII, gtrV, gtrX, oac, and wzx6 to identify S. flexneri serotypes 2a, 2b, 3a, 5a, 5b, 6, and X, and the other panel included ipaH, gtrI, gtrIc, and gtrIV to confirm Shigella detection and further identify S. flexneri serotypes 1a, 1b, 1d, 3b, 4a, 4b, 7a, and 7b. We first evaluated 283 Shigella isolates, and PCR serotyping demonstrated 97.0% (95% confidence interval, 93.0% to 99.0%) sensitivity and 99.9% (99.9% to 100%) specificity compared to conventional serotyping. The assays then were utilized on direct stool specimens. A quantitative detection algorithm was developed with a validation set of 174 Shigella culture-positive stool samples and further tested with a derivation set of 164 samples. The PCR serotyping on stool achieved 93% (89% to 96%) sensitivity and 99% (99% to 100%) specificity compared to serotyping. Most discrepancies were genotypic-phenotypic discordance, not genotypic failure. These real-time PCR assays provide an efficient and novel tool for S. flexneri serotype identification.en_US
dc.description.urihttps://doi.org/10.1128/JCM.02455-20en_US
dc.language.isoenen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.ispartofJournal of Clinical Microbiologyen_US
dc.rightsCopyright © 2021 Liu et al.en_US
dc.subjectPCRen_US
dc.subjectShigella flexnerien_US
dc.subjectserotypeen_US
dc.subjectstoolen_US
dc.titleEvaluation of Molecular Serotyping Assays for Shigella flexneri Directly on Stool Samples.en_US
dc.typeArticleen_US
dc.identifier.doi10.1128/JCM.02455-20
dc.identifier.pmid33239379
dc.source.volume59
dc.source.issue2
dc.source.countryUnited States


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