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dc.contributor.authorChattopadhyay, Suhana
dc.contributor.authorArnold, Justin D
dc.contributor.authorMalayil, Leena
dc.contributor.authorHittle, Lauren
dc.contributor.authorMongodin, Emmanuel F
dc.contributor.authorMarathe, Kalyani S
dc.contributor.authorGomez-Lobo, Veronica
dc.contributor.authorSapkota, Amy R
dc.date.accessioned2021-02-11T19:42:43Z
dc.date.available2021-02-11T19:42:43Z
dc.date.issued2021-01-14
dc.identifier.urihttp://hdl.handle.net/10713/14673
dc.description.abstractThe etiology of vulvar lichen sclerosus (LS) remains unclear; however, alterations in cutaneous and gut microbiota may be contributing to the pathogenesis of this inflammatory condition. To explore this hypothesis, we conducted a pilot case-control study, obtaining dermal swab and stool samples from prepubertal girls with vulvar LS (n = 5), girls with nonspecific vulvovaginitis (n = 5), and healthy controls (n = 3). Samples (n = 56) were subjected to total DNA extractions. Resulting DNA was purified, subjected to PCR (targeting the V3V4 region of the 16S rRNA gene), sequenced, and analyzed using QIIME, MetagenomeSeq, and DESeq2 software packages. Our findings showed that there were significant differences in the cutaneous and gut microbiotas of girls with LS compared to controls. On the skin, girls with LS had a statistically significantly higher relative abundance of Porphyromonas spp., Parvimonas spp., Peptoniphilus spp., Prevotella spp., Dialister spp., and Peptostreptococcus spp., but a lower relative abundance of Cornyebacterium compared to the control group. In the gut samples, girls with LS had a significantly higher relative abundance of Dialister spp., Clostridiales spp., Paraprevotella spp., Escherichia coli, Bifidobacterium adolescentis, and Akkermansia muciniphila, and a lower relative abundance of Roseburia faecis and Ruminococcus bromii compared to controls. These results suggest a potential association between cutaneous and gut dysbiosis and pediatric vulvar LS. Future studies involving larger samples sizes are warranted to further evaluate this association. © 2021 Chattopadhyay et al.en_US
dc.description.urihttps://doi.org/10.1371/journal.pone.0245243en_US
dc.language.isoenen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.ispartofPLoS ONEen_US
dc.subject.meshGastrointestinal Microbiomeen_US
dc.subject.meshSkin--microbiologyen_US
dc.subject.meshVulvar Lichen Sclerosis--etiologyen_US
dc.subject.meshVulvar Lichen Sclerosis--microbiologyen_US
dc.titlePotential role of the skin and gut microbiota in premenarchal vulvar lichen sclerosus: A pilot case-control studyen_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pone.0245243
dc.identifier.pmid33444404
dc.source.volume16
dc.source.issue1
dc.source.beginpagee0245243
dc.source.endpage
dc.source.countryUnited States


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