An in vivo atlas of host-pathogen transcriptomes during Streptococcus pneumoniae colonization and disease
Author
D'Mello, A.Riegler, A.N.
Martinez, E.
Beno, S.M.
Ricketts, T.D.
Foxman, E.F.
Orihuela, C.J.
Tettelin, H.
Date
2020-12-14Journal
Proceedings of the National Academy of Sciences of the United States of AmericaPublisher
Proceedings of the National Academy of Sciences of the United States of AmericaType
Article
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Streptococcus pneumoniae (Spn) colonizes the nasopharynx and can cause pneumonia. From the lungs it spreads to the bloodstream and causes organ damage. We characterized the in vivo Spn and mouse transcriptomes within the nasopharynx, lungs, blood, heart, and kidneys using three Spn strains. We identified Spn genes highly expressed at all anatomical sites and in an organ-specific manner; highly expressed genes were shown to have vital roles with knockout mutants. The in vivo bacterial transcriptome during colonization/disease was distinct from previously reported in vitro transcriptomes. Distinct Spn and host gene-expression profiles were observed during colonization and disease states, revealing specific genes/operons whereby Spn adapts to and influences host sites in vivo. We identified and experimentally verified host-defense pathways induced by Spn during invasive disease, including proinflammatory responses and the interferon response. These results shed light on the pathogenesis of Spn and identify therapeutic targets. Copyright Copyright 2020 the Author(s). Published by PNAS.Keyword
dual species RNA-seqhost-pathogen interactions
in vivo transcriptomics
mouse models of colonization and invasive disease
Streptococcus pneumoniae
Identifier to cite or link to this item
http://hdl.handle.net/10713/14524ae974a485f413a2113503eed53cd6c53
10.1073/pnas.2010428117
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