An in vivo atlas of host-pathogen transcriptomes during Streptococcus pneumoniae colonization and disease
JournalProceedings of the National Academy of Sciences of the United States of America
PublisherProceedings of the National Academy of Sciences of the United States of America
MetadataShow full item record
AbstractStreptococcus pneumoniae (Spn) colonizes the nasopharynx and can cause pneumonia. From the lungs it spreads to the bloodstream and causes organ damage. We characterized the in vivo Spn and mouse transcriptomes within the nasopharynx, lungs, blood, heart, and kidneys using three Spn strains. We identified Spn genes highly expressed at all anatomical sites and in an organ-specific manner; highly expressed genes were shown to have vital roles with knockout mutants. The in vivo bacterial transcriptome during colonization/disease was distinct from previously reported in vitro transcriptomes. Distinct Spn and host gene-expression profiles were observed during colonization and disease states, revealing specific genes/operons whereby Spn adapts to and influences host sites in vivo. We identified and experimentally verified host-defense pathways induced by Spn during invasive disease, including proinflammatory responses and the interferon response. These results shed light on the pathogenesis of Spn and identify therapeutic targets. Copyright Copyright 2020 the Author(s). Published by PNAS.
Keyworddual species RNA-seq
in vivo transcriptomics
mouse models of colonization and invasive disease
Identifier to cite or link to this itemhttp://hdl.handle.net/10713/14524
- Role of an iron-dependent transcriptional regulator in the pathogenesis and host response to infection with Streptococcus pneumoniae.
- Authors: Gupta R, Bhatty M, Swiatlo E, Nanduri B
- Issue date: 2013
- Nasopharyngeal colonization and invasive disease are enhanced by the cell wall hydrolases LytB and LytC of Streptococcus pneumoniae.
- Authors: Ramos-Sevillano E, Moscoso M, García P, García E, Yuste J
- Issue date: 2011
- Co-Transcriptomes of Initial Interactions In Vitro between Streptococcus Pneumoniae and Human Pleural Mesothelial Cells.
- Authors: Heath CJ, del Mar Cendra M, Watson A, Auger JP, Pandey A, Tighe P, Christodoulides M
- Issue date: 2015
- Co-colonization by Haemophilus influenzae with Streptococcus pneumoniae enhances pneumococcal-specific antibody response in young children.
- Authors: Xu Q, Pichichero ME
- Issue date: 2014 Feb 3
- Pneumococci in biofilms are non-invasive: implications on nasopharyngeal colonization.
- Authors: Gilley RP, Orihuela CJ
- Issue date: 2014