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    An in vivo atlas of host-pathogen transcriptomes during Streptococcus pneumoniae colonization and disease

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    Author
    D'Mello, A.
    Riegler, A.N.
    Martinez, E.
    Beno, S.M.
    Ricketts, T.D.
    Foxman, E.F.
    Orihuela, C.J.
    Tettelin, H.
    Date
    2020-12-14
    Journal
    Proceedings of the National Academy of Sciences of the United States of America
    Publisher
    Proceedings of the National Academy of Sciences of the United States of America
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.1073/pnas.2010428117
    Abstract
    Streptococcus pneumoniae (Spn) colonizes the nasopharynx and can cause pneumonia. From the lungs it spreads to the bloodstream and causes organ damage. We characterized the in vivo Spn and mouse transcriptomes within the nasopharynx, lungs, blood, heart, and kidneys using three Spn strains. We identified Spn genes highly expressed at all anatomical sites and in an organ-specific manner; highly expressed genes were shown to have vital roles with knockout mutants. The in vivo bacterial transcriptome during colonization/disease was distinct from previously reported in vitro transcriptomes. Distinct Spn and host gene-expression profiles were observed during colonization and disease states, revealing specific genes/operons whereby Spn adapts to and influences host sites in vivo. We identified and experimentally verified host-defense pathways induced by Spn during invasive disease, including proinflammatory responses and the interferon response. These results shed light on the pathogenesis of Spn and identify therapeutic targets. Copyright Copyright 2020 the Author(s). Published by PNAS.
    Keyword
    dual species RNA-seq
    host-pathogen interactions
    in vivo transcriptomics
    mouse models of colonization and invasive disease
    Streptococcus pneumoniae
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/14524
    ae974a485f413a2113503eed53cd6c53
    10.1073/pnas.2010428117
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