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dc.contributor.authorTran, Anh
dc.contributor.authorWan, Liting
dc.contributor.authorXu, Zhenbo
dc.contributor.authorHaro, Janette M
dc.contributor.authorLi, Bing
dc.contributor.authorJones, Jace W
dc.date.accessioned2021-01-26T14:44:21Z
dc.date.available2021-01-26T14:44:21Z
dc.date.issued2020-10-30
dc.identifier.urihttp://hdl.handle.net/10713/14413
dc.description.abstractSphingolipids have diverse structural and bioactive functions that play important roles in many key biological processes. Factors such as low relative abundance, varied structures, and a dynamic concentration range provide a difficult analytical challenge for sphingolipid detection. To further improve mass-spectrometry-based sphingolipid analysis, lithium adduct consolidation was implemented to decrease spectral complexity and combine signal intensities, leading to increased specificity and sensitivity. We report the use of lithium hydroxide as a base in a routine hydrolysis procedure in order to effectively remove common ionization suppressants (such as glycolipids and glycerophospholipids) and introduce a source of lithium into the sample. In conjunction, an optimized MALDI matrix system, featuring 2',4',6'-trihydroxyacetophenone (THAP) is used to facilitate lithium adduct consolidation during the MALDI process. The result is a robust and high-throughput sphingolipid detection scheme, particularly of low-abundance ceramides. Application of our developed workflow includes the detection of differentially expressed liver sphingolipid profiles from a high-fat-induced obesity mouse model. We also demonstrate the method's effectiveness in detecting various sphingolipids in brain and plasma matrices. These results were corroborated with data from UHPLC HR MS/MS and MALDI FT-ICR, verifying the efficacy of the method application. Overall, we demonstrate a high-throughput workflow for sphingolipid analysis in various biological matrices by the use of MALDI TOF and lithium adduct consolidation.en_US
dc.description.urihttps://doi.org/10.1021/jasms.0c00322en_US
dc.language.isoenen_US
dc.publisherAmerican Chemical Societyen_US
dc.relation.ispartofJournal of the American Society for Mass Spectrometryen_US
dc.subjectMALDI TOF mass spectrometryen_US
dc.subjectbase hydrolysisen_US
dc.subjectlithium adductionen_US
dc.subjectsphingolipidsen_US
dc.titleLithium Hydroxide Hydrolysis Combined with MALDI TOF Mass Spectrometry for Rapid Sphingolipid Detection.en_US
dc.typeArticleen_US
dc.identifier.doi10.1021/jasms.0c00322
dc.identifier.pmid33124427
dc.source.volume32
dc.source.issue1
dc.source.beginpage289
dc.source.endpage300
dc.source.countryUnited States
dc.source.countryUnited States


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