Show simple item record

dc.contributor.authorBarry, Kathryn Hughes
dc.contributor.authorMohanty, Kareshma
dc.contributor.authorErickson, Patricia A
dc.contributor.authorWang, Difei
dc.contributor.authorShi, Jianxin
dc.contributor.authorRose, Gary
dc.contributor.authorCellini, Ashley
dc.contributor.authorClark, Kimberly
dc.contributor.authorAmbulos, Nicholas
dc.contributor.authorYin, Jing
dc.contributor.authorYan, Liying
dc.contributor.authorPoulin, Matthew
dc.contributor.authorMeyer, Ann
dc.contributor.authorZhang, Yuji
dc.contributor.authorBentzen, Søren M
dc.contributor.authorBurke, Allen
dc.contributor.authorHussain, Arif
dc.contributor.authorBerndt, Sonja I
dc.date.accessioned2021-01-11T19:22:23Z
dc.date.available2021-01-11T19:22:23Z
dc.date.issued2020-12-24
dc.identifier.urihttp://hdl.handle.net/10713/14321
dc.description.abstractIncreasing evidence suggests a role of epigenetic mechanisms at chromosome 8q24, an important cancer genetic susceptibility region, in prostate cancer. We investigated whether MYC DNA methylation at 8q24 (six CpG sites from exon 3 to the 3' UTR) in prostate tumor was associated with tumor aggressiveness (based on Gleason score, GS), and we incorporated RNA expression data to investigate the function. We accessed radical prostatectomy tissue for 50 Caucasian and 50 African American prostate cancer patients at the University of Maryland Medical Center, selecting an equal number of GS 6 and GS 7 cases per group. MYC DNA methylation was lower in tumor than paired normal prostate tissue for all six CpG sites (median difference: -14.74 to -0.20 percentage points), and we observed similar results for two nearby sites in The Cancer Genome Atlas (p < 0.0001). We observed significantly lower methylation for more aggressive (GS 7) than less aggressive (GS 6) tumors for three exon 3 sites (for CpG 212 (chr8:128753145), GS 6 median = 89.7%; GS 7 median = 85.8%; p-value = 9.4 × 10-4). MYC DNA methylation was not associated with MYC expression, but was inversely associated with PRNCR1 expression after multiple comparison adjustment (q-value = 0.04). Findings suggest that prostate tumor MYC exon 3 hypomethylation is associated with increased aggressiveness.en_US
dc.description.urihttps://doi.org/10.3390/genes12010012en_US
dc.language.isoenen_US
dc.publisherMDPI AGen_US
dc.relation.ispartofGenesen_US
dc.subjectDNA methylationen_US
dc.subjectGleason score (GS)en_US
dc.subjectMYCen_US
dc.subjectRNA expressionen_US
dc.subjectaggressive diseaseen_US
dc.subjectnon-coding RNAs (ncRNAs)en_US
dc.subjectprostate canceren_US
dc.subjecttumor tissue biomarkersen_US
dc.titleDNA Methylation in Prostate Tumor Tissue Is Associated with Gleason Scoreen_US
dc.typeArticleen_US
dc.identifier.doi10.3390/genes12010012
dc.identifier.pmid33374332
dc.source.volume12
dc.source.issue1
dc.source.countryUnited States
dc.source.countrySwitzerland


This item appears in the following Collection(s)

Show simple item record