• Login
    View Item 
    •   UMB Digital Archive
    • School, Graduate
    • Theses and Dissertations All Schools
    • View Item
    •   UMB Digital Archive
    • School, Graduate
    • Theses and Dissertations All Schools
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UMB Digital ArchiveCommunitiesPublication DateAuthorsTitlesSubjectsThis CollectionPublication DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    Display statistics

    Cloning and molecular characterization of the murine S100(beta), a glial-specific neurotrophic factor in the nervous system.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Find Full text
    Author
    Jiang, Hao
    Advisor
    Hilt, Dana C.
    Date
    1994
    Type
    dissertation
    
    Metadata
    Show full item record
    Abstract
    The organization, sequence and transcriptional regulation of the murine S100{dollar}\beta{dollar} gene have been studied. The gene is approximately 9 kb in length and is composed of three exons and two introns. The murine S100{dollar}\beta{dollar} gene contains a TATA box (AATAA) and a reverse CCAAT box (ATTGG) located at 30 nucleotides and 92 nucleotides upstream of the transcription start site, respectively. A 149 bp DNA fragment ({dollar}-{dollar}157/{dollar}-{dollar}9) spanning the TATA box and reverse CCAAT box functions as a promoter. The murine S100{dollar}\beta{dollar} promoter drives a 4-fold higher level of transcription in C6 rat glioma cells than in non-glial 3T3 murine fibroblast cells, suggesting the existence of a cell type-specific regulatory element within the promoter region. The 5{dollar}\sp\prime{dollar}-flanking region suppresses transcription from the homologous S100{dollar}\beta{dollar} as well as heterologous SV40 promoters in an orientation-independent fashion. However, the 5{dollar}\sp\prime{dollar}-flanking region exhibits cell-type specificity when suppressing the S100{dollar}\beta{dollar} promoter-dependent transcription, indicating its involvement in the regulation of the cell type-specific expression of the murine S100{dollar}\beta{dollar} gene. In order to map the cell type-specific regulatory elements, transcriptional analyses of various deletions of the 5{dollar}\sp\prime{dollar}-flanking region were carried out in both C6 and 3T3 cells. Two cell type-specific negative regulatory elements, one active in non-glial cells and another active in glial cells, were mapped to the regions {dollar}-{dollar}1552/{dollar}-{dollar}1234 and {dollar}-{dollar}1234/{dollar}-{dollar}551, respectively. A strong negative regulatory element and a relatively weak negative regulatory element were located in the regions {dollar}-{dollar}551/{dollar}-{dollar}157 and {dollar}-{dollar}1669/{dollar}-{dollar}1552, respectively. These results lead to the conclusion that the murine S100{dollar}\beta{dollar} gene is under complex transcriptional regulation involving tonic negative control exerted by a combination of multiple cis-acting regulatory elements including cell type-specific elements. Gel mobility shift assay with the cell type-specific regulatory element III (CSREIII, {dollar}-{dollar}1552/{dollar}-{dollar}1234) showed that a unique trans-acting protein factor may be present in 3T3 nuclear extract but absent in C6 nuclear extract. This factor can specifically bind to a 17 bp DNA fragment ({dollar}-{dollar}1513/{dollar}-{dollar}1497) which contains a consensus silencer element. These findings suggest that the glial-specific expression of the murine S100{dollar}\beta{dollar} gene is probably mediated by both cis-acting silencer elements and trans-acting protein factors.
    Description
    University of Maryland, Baltimore. Ph.D. 1994
    Keyword
    Biology, Molecular
    Biology, Neuroscience
    glial-specific neurotrophic factor
    s100 beta
    Mice
    S100 Proteins
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/1427
    Collections
    Theses and Dissertations All Schools

    entitlement

     
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Policies | Contact Us | UMB Health Sciences & Human Services Library
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.