• Login
    View Item 
    •   UMB Digital Archive
    • UMB Open Access Articles
    • UMB Open Access Articles
    • View Item
    •   UMB Digital Archive
    • UMB Open Access Articles
    • UMB Open Access Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UMB Digital ArchiveCommunitiesPublication DateAuthorsTitlesSubjectsThis CollectionPublication DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    Display statistics

    Optogenetic activation of muscle contraction .

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Author
    Ganji, Elahe
    Chan, C Savio
    Ward, Christopher W
    Killian, Megan L
    Date
    2020-08-10
    Journal
    Connective Tissue Research
    Publisher
    Taylor and Francis Inc.
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.1080/03008207.2020.1798943
    Abstract
    Purpose: Optogenetics is an emerging alternative to traditional electrical stimulation to initiate action potentials in activatable cells both ex vivo and in vivo. Optogenetics has been commonly used in mammalian neurons and more recently, it has been adapted for activation of cardiomyocytes and skeletal muscle. Therefore, the aim of this study was to evaluate the stimulation feasibility and sustain isometric muscle contraction and limit decay for an extended period of time (1s), using non-invasive transdermal light activation of skeletal muscle (triceps surae) in vivo. Materials and Methods: We used inducible Cre recombination to target expression of Channelrhodopsin-2 (ChR2(H134R)-EYFP) in skeletal muscle (Acta1-Cre) in mice. Fluorescent imaging confirmed that ChR2 expression is localized in skeletal muscle and does not have specific expression in sciatic nerve branch, therefore, allowing for non-nerve mediated optical stimulation of skeletal muscle. We induced muscle contraction using transdermal exposure to blue light and selected 10 Hz stimulation after controlled optimization experiments to sustain prolonged muscle contraction. Results: Increasing the stimulation frequency from 10 Hz to 40 Hz increased the muscle contraction decay during prolonged 1s stimulation, highlighting frequency dependency and importance of membrane repolarization for effective light activation. Finally, we showed that optimized pulsed optogenetic stimulation of 10 Hz resulted in comparable ankle torque and contractile functionality to that of electrical stimulation. Conclusions: Our results demonstrate the feasibility and repeatability of non-invasive optogenetic stimulation of muscle in vivo and highlight optogenetic stimulation as a powerful tool for non-invasive in vivo direct activation of skeletal muscle.
    Sponsors
    Eunice Kennedy Shriver National Institute of Child Health and Human Development (R03 HD094594 to MLK) and The National Institute of Neurological Disorders and Stroke (R01 NS069777 to CSC).
    Keyword
    Optogenetics
    channelrhodopsin-2
    cre-lox
    skeletal muscle
    triceps surae
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/14236
    ae974a485f413a2113503eed53cd6c53
    10.1080/03008207.2020.1798943
    Scopus Count
    Collections
    UMB Open Access Articles

    entitlement

    Related articles

    • Optogenetic control of contractile function in skeletal muscle.
    • Authors: Bruegmann T, van Bremen T, Vogt CC, Send T, Fleischmann BK, Sasse P
    • Issue date: 2015 Jun 2
    • Optogenetic analysis of neuromuscular transmission in the colon of ChAT-ChR2-YFP BAC transgenic mice.
    • Authors: Perez-Medina AL, Galligan JJ
    • Issue date: 2019 Nov 1
    • Direct optogenetic stimulation of smooth muscle cells to control gastric contractility.
    • Authors: Vogt M, Schulz B, Wagdi A, Lebert J, van Belle GJ, Christoph J, Bruegmann T, Patejdl R
    • Issue date: 2021
    • Optogenetic Modulation of Urinary Bladder Contraction for Lower Urinary Tract Dysfunction.
    • Authors: Park JH, Hong JK, Jang JY, An J, Lee KS, Kang TM, Shin HJ, Suh JF
    • Issue date: 2017 Jan 18
    • Spot light on skeletal muscles: optogenetic stimulation to understand and restore skeletal muscle function.
    • Authors: van Bremen T, Send T, Sasse P, Bruegmann T
    • Issue date: 2017 Aug
    DSpace software (copyright © 2002 - 2022)  DuraSpace
    Quick Guide | Policies | Contact Us | UMB Health Sciences & Human Services Library
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.