Potential caveats of putative microglia-specific markers for assessment of age-related cerebrovascular neuroinflammation.
Author
Honarpisheh, PedramLee, Juneyoung
Banerjee, Anik
Blasco-Conesa, Maria P
Honarpisheh, Parisa
d'Aigle, John
Mamun, Abdullah A
Ritzel, Rodney M
Chauhan, Anjali
Ganesh, Bhanu P
McCullough, Louise D
Date
2020-12-01Journal
Journal of NeuroinflammationPublisher
Springer NatureType
Article
Metadata
Show full item recordAbstract
We demonstrate that Tmem119 and P2RY12 expression is evident within both CD45int and CD45high myeloid populations in models of stroke, CAA, and aging. Interestingly, LPS stimulation of FACS-sorted adult microglia suggested that these brain-resident myeloid cells can upregulate CD45 and downregulate Tmem119 and P2RY12, making them indistinguishable from peripherally derived myeloid populations. Importantly, our findings show that these changes in the molecular signatures of microglia can occur without a contribution from the other brain-resident or peripherally sourced immune cells.Keyword
AgingBrain infiltrating myeloid cells
CD45
Cerebral amyloid angiopathy
Microglia
Neuroinflammation
P2RY12
Stroke
Tmem119
Identifier to cite or link to this item
http://hdl.handle.net/10713/14204ae974a485f413a2113503eed53cd6c53
10.1186/s12974-020-02019-5
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