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    Antimicrobial peptide activity is anticorrelated with lipid a leaflet affinity

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    Author
    Nelson, Nathaniel
    Opene, Belita
    Ernst, Robert K
    Schwartz, Daniel K
    Date
    2020-11-30
    Journal
    PLoS ONE
    Publisher
    Public Library of Science
    Type
    Article
    
    Metadata
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    See at
    https://doi.org/10.1371/journal.pone.0242907
    Abstract
    The activity of antimicrobial peptides (AMPs) has significant bacterial species bias, the mechanisms of which are not fully understood. We employed single-molecule tracking to measure the affinity of three different AMPs to hybrid supported bilayers composed of lipid A extracted from four different Gram negative bacteria and observed a strong empirical anticorrelation between the affinity of a particular AMP to a given lipid A layer and the activity of that AMP towards the bacterium from which that lipid A was extracted. This suggested that the species bias of AMP activity is directly related to AMP interactions with bacterial outer membranes, despite the fact that the mechanism of antimicrobial activity occurs at the inner membrane. The trend also suggested that the interactions between AMPs and the outer membrane lipid A (even in the absence of other components, such as lipopolysaccharides) capture effects that are relevant to the minimum inhibitory concentration.
    Keyword
    antimicrobial peptides
    bacterial species bias
    lipid A layer
    Pore Forming Cytotoxic Proteins
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/14199
    ae974a485f413a2113503eed53cd6c53
    10.1371/journal.pone.0242907
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