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    Transcriptome dataset of Babesia bovis life stages within vertebrate and invertebrate hosts

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    Author
    Ueti, Massaro W.
    Johnson, Wendell C.
    Kappmeyer, Lowell S.
    Herndon, David R.
    Mousel, Michelle R.
    Reif, Kathryn E.
    Taus, Naomi S.
    Ifeonu, Olukemi O.
    Silva, Joana C.
    Suarez, Carlos E.
    Brayton, Kelly A.
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    Date
    2020-12-01
    Journal
    Data in Brief
    Publisher
    Elsevier Ltd.
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.1016/j.dib.2020.106533
    Abstract
    Babesia bovis is a hemoprotozoan parasite of cattle that has a complex life cycle within vertebrate and invertebrate hosts. In the mammalian host, B. bovis undergoes asexual reproduction while in the tick midgut, gametes are induced, fuse, and form zygotes. The zygote infects tick gut epithelial cells and transform into kinetes that are released into the hemolymph and invade other tick tissues such as the ovaries, resulting in transovarial transmission to tick offspring. To compare gene regulation between different B. bovis life stages, we collected parasites infecting bovine erythrocytes and tick hemolymph. Total RNA samples were isolated, and multiplexed libraries sequenced using paired-end 100 cycle reads of a HiSeq 2500. The data was normalized using the TMM method and analysed for significant differential expression using the generalized linear model likelihood ratio test (GLM LRT) in edgeR. To validate our datasets, ten genes were selected using NormFinder. Genes that had no significant fold change between the blood and tick stages in the RNA-Seq datasets were tested by quantitative PCR to determine their suitability as “housekeeping” genes. The normalized RNA-Seq data revealed genes upregulated during infection of the mammalian host or tick vector and six upregulated genes were validated by quantitative PCR. These datasets can help identify useful targets for controlling bovine babesiosis.
    Sponsors
    United States Agency for International Development
    Keyword
    Babesia
    Bovine
    Bovine babesiosis
    Gene expression
    Kinetes
    Rhipicephalus microplus
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/14176
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.dib.2020.106533
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