Rapid, Ultrasensitive, and Quantitative Detection of SARS-CoV-2 Using Antisense Oligonucleotides Directed Electrochemical Biosensor Chip.
Date
2020-10-20Journal
ACS NanoPublisher
American Chemical SocietyType
Article
Metadata
Show full item recordAbstract
A large-scale diagnosis of the severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) is essential to downregulate its spread within as well as across communities and mitigate the current outbreak of the pandemic novel coronavirus disease 2019 (COVID-19). Herein, we report the development of a rapid (less than 5 min), low-cost, easy-to-implement, and quantitative paper-based electrochemical sensor chip to enable the digital detection of SARS-CoV-2 genetic material. The biosensor uses gold nanoparticles (AuNPs), capped with highly specific antisense oligonucleotides (ssDNA) targeting viral nucleocapsid phosphoprotein (N-gene). The sensing probes are immobilized on a paper-based electrochemical platform to yield a nucleic-acid-testing device with a readout that can be recorded with a simple hand-held reader. The biosensor chip has been tested using samples collected from Vero cells infected with SARS-CoV-2 virus and clinical samples. The sensor provides a significant improvement in output signal only in the presence of its target-SARS-CoV-2 RNA-within less than 5 min of incubation time, with a sensitivity of 231 (copies μL-1)-1 and limit of detection of 6.9 copies/μL without the need for any further amplification. The sensor chip performance has been tested using clinical samples from 22 COVID-19 positive patients and 26 healthy asymptomatic subjects confirmed using the FDA-approved RT-PCR COVID-19 diagnostic kit. The sensor successfully distinguishes the positive COVID-19 samples from the negative ones with almost 100% accuracy, sensitivity, and specificity and exhibits an insignificant change in output signal for the samples lacking a SARS-CoV-2 viral target segment (e.g., SARS-CoV, MERS-CoV, or negative COVID-19 samples collected from healthy subjects). The feasibility of the sensor even during the genomic mutation of the virus is also ensured from the design of the ssDNA-conjugated AuNPs that simultaneously target two separate regions of the same SARS-CoV-2 N-gene.Identifier to cite or link to this item
http://hdl.handle.net/10713/14145ae974a485f413a2113503eed53cd6c53
10.1021/acsnano.0c06392
Scopus Count
Related articles
- Selective Naked-Eye Detection of SARS-CoV-2 Mediated by N Gene Targeted Antisense Oligonucleotide Capped Plasmonic Nanoparticles.
- Authors: Moitra P, Alafeef M, Dighe K, Frieman MB, Pan D
- Issue date: 2020 Jun 23
- Ultrasensitive supersandwich-type electrochemical sensor for SARS-CoV-2 from the infected COVID-19 patients using a smartphone.
- Authors: Zhao H, Liu F, Xie W, Zhou TC, OuYang J, Jin L, Li H, Zhao CY, Zhang L, Wei J, Zhang YP, Li CP
- Issue date: 2021 Jan 15
- Rapid electrochemical detection of coronavirus SARS-CoV-2.
- Authors: Chaibun T, Puenpa J, Ngamdee T, Boonapatcharoen N, Athamanolap P, O'Mullane AP, Vongpunsawad S, Poovorawan Y, Lee SY, Lertanantawong B
- Issue date: 2021 Feb 5
- Development and Clinical Application of a Rapid and Sensitive Loop-Mediated Isothermal Amplification Test for SARS-CoV-2 Infection.
- Authors: Hu X, Deng Q, Li J, Chen J, Wang Z, Zhang X, Fang Z, Li H, Zhao Y, Yu P, Li W, Wang X, Li S, Zhang L, Hou T
- Issue date: 2020 Aug 26
- Rapid Detection of IgM Antibodies against the SARS-CoV-2 Virus via Colloidal Gold Nanoparticle-Based Lateral-Flow Assay.
- Authors: Huang C, Wen T, Shi FJ, Zeng XY, Jiao YJ
- Issue date: 2020 Jun 2