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dc.contributor.authorChen, Hong
dc.contributor.authorYang, Hui
dc.contributor.authorWeir, Michael D.
dc.contributor.authorSchneider, Abraham
dc.contributor.authorRen, Ke
dc.contributor.authorHomayounfar, Negar
dc.contributor.authorOates, Thomas W.
dc.contributor.authorZhang, Ke
dc.contributor.authorLiu, Jin
dc.contributor.authorHu, Tao
dc.contributor.authorXu, Hockin H.K.
dc.date.accessioned2020-11-20T20:26:00Z
dc.date.available2020-11-20T20:26:00Z
dc.date.issued2020-11-01
dc.identifier.urihttp://hdl.handle.net/10713/14135
dc.description.abstractOsteomyelitis and post-operative infections are major problems in orthopedic, dental and craniofacial surgeries. It is highly desirable for a tissue engineering construct to kill bacteria, while simultaneously delivering stem cells and enhancing cell function and tissue regeneration. The objectives of this study were to: (1) develop a novel injectable calcium phosphate cement (CPC) scaffold containing antibiotic ornidazole (ORZ) while encapsulating human periodontal ligament stem cells (hPDLSCs), and (2) investigate the inhibition efficacy against Staphylococcus aureus (S. aureus) and the promotion of hPDLSC function for osteogenesis for the first time. ORZ was incorporated into a CPC-chitosan scaffold. hPDLSCs were encapsulated in alginate microbeads (denoted hPDLSCbeads). The ORZ-loaded CPCC+hPDLSCbeads scaffold was fully injectable, and had a flexural strength of 3.50 ± 0.92 MPa and an elastic modulus of 1.30 ± 0.45 GPa, matching those of natural cancellous bone. With 6 days of sustained ORZ release, the CPCC+10ORZ (10% ORZ) scaffold had strong antibacterial effects on S. aureus, with an inhibition zone of 12.47 ± 1.01 mm. No colonies were observed in the CPCC+10ORZ group from 3 to 7 days. ORZ-containing scaffolds were biocompatible with hPDLSCs. CPCC+10ORZ+hPDLSCbeads scaffold with osteogenic medium had 2.4-fold increase in alkaline phosphatase (ALP) activity and bone mineral synthesis by hPDLSCs, as compared to the control group (p < 0.05). In conclusion, the novel antibacterial construct with stem cell delivery had injectability, good strength, strong antibacterial effects and biocompatibility, supporting osteogenic differentiation and bone mineral synthesis of hPDLSCs. The injectable and mechanically-strong CPCC+10ORZ+hPDLSCbeads construct has great potential for treating bone infections and promoting bone regeneration.en_US
dc.description.urihttps://doi.org/10.1039/d0ra06873jen_US
dc.language.isoenen_US
dc.publisherRoyal Society of Chemistryen_US
dc.relation.ispartofRSC Advancesen_US
dc.subjectbone tissue engineeringen_US
dc.subject.meshCalcium Phosphatesen_US
dc.subject.meshTissue Scaffoldsen_US
dc.subject.meshTissue Engineeringen_US
dc.subject.meshOsteomyelitisen_US
dc.subject.meshPeriodontal Ligamenten_US
dc.subject.meshStem Cellsen_US
dc.titleAn antibacterial and injectable calcium phosphate scaffold delivering human periodontal ligament stem cells for bone tissue engineeringen_US
dc.typeArticleen_US
dc.identifier.doi10.1039/d0ra06873j
dc.source.journaltitleRSC Advances
dc.source.volume10
dc.source.issue66
dc.source.beginpage40157
dc.source.endpage40170


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