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dc.contributor.authorUeti, Massaro W
dc.contributor.authorJohnson, Wendell C
dc.contributor.authorKappmeyer, Lowell S
dc.contributor.authorHerndon, David R
dc.contributor.authorMousel, Michelle R
dc.contributor.authorReif, Kathryn E
dc.contributor.authorTaus, Naomi S
dc.contributor.authorIfeonu, Olukemi O
dc.contributor.authorSilva, Joana C
dc.contributor.authorSuarez, Carlos E
dc.contributor.authorBrayton, Kelly A
dc.date.accessioned2020-11-18T19:14:26Z
dc.date.available2020-11-18T19:14:26Z
dc.date.issued2020-10-15
dc.identifier.urihttp://hdl.handle.net/10713/14120
dc.description.abstractThroughout their life cycle, Babesia parasites alternate between a mammalian host, where they cause babesiosis, and the tick vector. Transition between hosts results in distinct environmental signals that influence patterns of gene expression, consistent with the morphological and functional changes operating in the parasites during their life stages. In addition, comparing differential patterns of gene expression among mammalian and tick parasite stages can provide clues for developing improved methods of control. Hereby, we upgraded the genome assembly of Babesia bovis, a bovine hemoparasite, closing a 139 kbp gap, and used RNA-Seq datasets derived from mammalian blood and tick kinete stages to update the genome annotation. Of the originally annotated genes, 1,254 required structural changes, and 326 new genes were identified, leading to a different predicted proteome compared to the original annotation. Next, the RNA-Seq data was used to identify B. bovis genes that were differentially expressed in the vertebrate and arthropod hosts. In blood stages, 28% of the genes were upregulated up to 300 fold, whereas 26% of the genes in kinetes, a tick stage, were upregulated up to >19,000 fold. We thus discovered differentially expressed genes that may play key biological roles and serve as suitable targets for the development of vaccines to control bovine babesiosis.en_US
dc.description.urihttps://doi.org/10.1016/j.ijpara.2020.08.006en_US
dc.language.isoenen_US
dc.publisherElsevier Ltd.en_US
dc.relation.ispartofInternational Journal for Parasitologyen_US
dc.rightsCopyright © 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved.en_US
dc.subjectBabesia bovisen_US
dc.subjectBovine babesiosisen_US
dc.subjectDifferential gene expressionen_US
dc.subjectGenome annotationen_US
dc.subjectKinetesen_US
dc.subjectRNA-Seqen_US
dc.titleComparative analysis of gene expression between Babesia bovis blood stages and kinetes allowed by improved genome annotationen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.ijpara.2020.08.006
dc.identifier.pmid33069745
dc.source.countryEngland


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