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    Comparative analysis of gene expression between Babesia bovis blood stages and kinetes allowed by improved genome annotation

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    Author
    Ueti, Massaro W
    Johnson, Wendell C
    Kappmeyer, Lowell S
    Herndon, David R
    Mousel, Michelle R
    Reif, Kathryn E
    Taus, Naomi S
    Ifeonu, Olukemi O
    Silva, Joana C
    Suarez, Carlos E
    Brayton, Kelly A
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    Date
    2020-10-15
    Journal
    International Journal for Parasitology
    Publisher
    Elsevier Ltd.
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.1016/j.ijpara.2020.08.006
    Abstract
    Throughout their life cycle, Babesia parasites alternate between a mammalian host, where they cause babesiosis, and the tick vector. Transition between hosts results in distinct environmental signals that influence patterns of gene expression, consistent with the morphological and functional changes operating in the parasites during their life stages. In addition, comparing differential patterns of gene expression among mammalian and tick parasite stages can provide clues for developing improved methods of control. Hereby, we upgraded the genome assembly of Babesia bovis, a bovine hemoparasite, closing a 139 kbp gap, and used RNA-Seq datasets derived from mammalian blood and tick kinete stages to update the genome annotation. Of the originally annotated genes, 1,254 required structural changes, and 326 new genes were identified, leading to a different predicted proteome compared to the original annotation. Next, the RNA-Seq data was used to identify B. bovis genes that were differentially expressed in the vertebrate and arthropod hosts. In blood stages, 28% of the genes were upregulated up to 300 fold, whereas 26% of the genes in kinetes, a tick stage, were upregulated up to >19,000 fold. We thus discovered differentially expressed genes that may play key biological roles and serve as suitable targets for the development of vaccines to control bovine babesiosis.
    Rights/Terms
    Copyright © 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved.
    Keyword
    Babesia bovis
    Bovine babesiosis
    Differential gene expression
    Genome annotation
    Kinetes
    RNA-Seq
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/14120
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.ijpara.2020.08.006
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    UMB Open Access Articles 2020

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